Abstract: Clonorchis sinensis primarily lives in the host liver and biliary system, leading to serious health issues, such as hepatobiliary lesions and cholangiocarcinoma. Establishment of rapid, sensitive and specific tools is of great significance for early diagnosis and management of clonorchiasis. Recently, molecular biological techniques have been widely used for detection of C. sinensis infections. This review summarizes the advances in the major molecular assays for detection of C. sinensis infections in intermediate and definite hosts, including PCR, loop⁃mediated isothermal amplification, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR⁃associated proteins system, and high⁃throughput sequencing, and summarizes the characteristics and application prospects of these assays, so as to provide precision detection and management of C. sinensis infection.

Key words: Clonorchis sinensis, Clonorchiasis, Molecular diagnosis, Detection technology

摘要： 华支睾吸虫主要寄生于宿主肝脏和胆道系统，可导致肝胆损伤甚至肝胆管癌等严重后果。建立快速、敏感和特异的检测方法对于疾病早期诊断与防控具有重要意义。近年来，分子生物学技术在华支睾吸虫感染检测中得到广泛应用。本文综述了华支睾吸虫中间宿主及终宿主感染的主要分子检测技术进展，包括PCR、环介导等温核酸扩增、规律成簇间隔短回文重复序列相关蛋白系统及高通量测序等技术，并对不同技术的特点及应用前景进行了总结，以期为华支睾吸虫感染精准检测与防控提供参考。

关键词: 华支睾吸虫, 华支睾吸虫病, 分子诊断, 检测技术 ,