Abstract: Objective　To investigate the prevalence of pinworm infections among kindergarten children and the effectiveness of health interventions among teachers and parents in Gaoyou City, so as to provide insights into optimization of enterobiasis control strategies.  Methods　From September to November each year from 2023 to 2025, Gaoyou City's 57 kindergartens were divided into five zones based on the five cardinal directions: east, west, south, north, and center. At least one kindergarten was randomly selected as a survey site in each zone, with a minimum of 200 children surveyed per site. The transparent adhesive tape anal swab method was used to detect pinworm infection in the surveyed children, while the transparent adhesive tape sticking method was employed to assess pinworm egg contamination in the children's home and classroom environments. Concurrently, health education interventions were conducted for kindergarten teachers and parents. Additionally, self⁃administered questionnaires were administered to teachers and parents to evaluate children's hygiene behaviors and the awareness rates of pinworm prevention knowledge among teachers and parents before and after the health education interventions.  Results　From 2023 to 2025, a total of 1 939 children from nine kindergartens in Gaoyou City were tested for pinworm infection, with two cases detected, resulting in an overall infection rate of 0.10% (2/1 939). No pinworm eggs were found in the samples from the children's families or classroom environments. In public kindergartens, the proportions of children practicing the four hygiene habits, namely "handwashing after defecation" [96.10% (1 305/1 358) vs. 74.65% (212/284); [χ2] = 153.646, P < 0.05], "regular nail trimming" [92.93%(1 262/1 358) vs. 77.82% (221/284); [χ2] = 61.349, P < 0.05], "not biting toys or pencils" [88.14% (1 197/1 358) vs. 76.76% (218/284); [χ2] = 25.549, P < 0.05], and "showering twice or more per week" [89.03% (1 209/1 358) vs. 74.65% (212/284); [χ2] = 41.699, P < 0.05], were all higher than those in private kindergartens. Before health education intervention, the awareness rates of pinworm prevention knowledge among teachers in public and private kindergartens were 86.89% (53/61) and 75.00% (21/28), respectively, with no statistically significant difference ([χ2] = 1.935, P > 0.05). The awareness rates among parents of children in public and private kindergartens were 64.73% (879/1 358) and 57.39% (163/284), respectively, showing a statistically significant difference ([χ2] = 5.447, P < 0.05). After the intervention, the awareness rates among teachers in public and private kindergartens increased to 96.72% (59/61) and 82.14% (23/28), respectively, while those among parents rose to 88.95% (1 208/1 358) and 77.81% (221/284), both with statistically significant differences ([χ2] = 5.628 and 25.808, both P < 0.05). Among the questionnaire items, teachers [32.58% (29/89)] and parents [26.00% (427/1 642)] had the lowest overall awareness rate regarding the "main reasons for the difficulty in preventing and controlling pinworm disease". After the intervention, these rates increased to 77.53% (69/89) and 70.77% (1 162/1 642), respectively. Conclusions　The prevalence of pinworm infection in kindergarten children in Gaoyou City is at a low level. Health intervention can effectively improve the knowledge level of teachers and parents on the prevention and treatment of pinworm disease, but further efforts are needed to strengthen the health education and management of pinworm disease in private kindergartens.

Key words: Enterobius vermicularis, Infection rate, Preschool, Children, Health education, Awareness rate, Gaoyou City

摘要： 目的　了解高邮市幼儿园儿童蛲虫感染现状及教师和家长的健康干预效果，为优化蛲虫病防控策略提供科学依据。方法　2023—2025年，每年9—11月按东、西、南、北、中5个方位将高邮市57所幼儿园分成5个片区，每个片区随机抽取至少1所幼儿园作为调查点，每个调查点至少调查200名儿童。对调查儿童采用透明胶纸肛拭法检测蛲虫感染情况，对儿童所在家庭及班级环境采用透明胶纸粘贴法检测蛲虫卵污染情况。同时，对幼儿园教师及儿童家长开展健康教育干预，并采用自填式问卷进行调查，以了解儿童卫生行为习惯及健康教育干预前后教师与家长蛲虫病防治知识知晓率。结果　2023—2025年累计对高邮市9所幼儿园的1 939名儿童进行蛲虫感染检测，检出蛲虫感染2例，总感染率为0.10%（2/1 939）。所有儿童家庭及班级环境样本中均未检出蛲虫卵。公立幼儿园中具有“便后洗手”［96.10%（1 305/ 1 358）vs. 74.65%（212/284）；[χ2] = 153.646，P < 0.05］、“勤剪指甲”［92.93%（1 262/1 358）vs. 77.82%（221/284）；[χ2] = 61.349，P < 0.05］、“不咬玩具或铅笔”［88.14%（1 197/1 358）vs. 76.76%（218/284）；[χ2] = 25.549，P < 0.05］、“每周洗澡2次及以上”［89.03%（1 209/1 358）vs. 74.65%（212/284）； [χ2] = 41.699，P < 0.05］4项卫生行为习惯的儿童占比均高于私立幼儿园。健康教育干预前，公立和私立幼儿园教师蛲虫病防治知识知晓率分别为86.89%（53/61）和75.00%（21/28），差异无统计学意义（[χ2]  = 1.935，P > 0.05）；家长防治知识知晓率分别为64.73%（879/1 358）和57.39%（163/284），差异有统计学意义（[χ2] = 5.447，P < 0.05）。干预后，公立和私立幼儿园教师蛲虫病防治知识知晓率分别提升至96.72%（59/61）和82.14%（23/28），家长知晓率分别提升至88.95%（1 208/1 358）和77.81%（221/284），差异均有统计学意义（[χ2] = 5.628、25.808，P均< 0.05）。在调查问卷各条目中，教师［32.58%（29/89）］和家长［26.00%（427/1 642）］对“蛲虫病难防治的主要原因”的总体知晓率均最低，干预后知晓率分别提升至77.53%（69/89）和70.77%（1 162/1 642）。结论　高邮市幼儿园儿童蛲虫感染处于低流行水平。健康干预可有效提升教师和家长的蛲虫病防治知识水平，但仍需进一步加强私立幼儿园蛲虫病健康教育与管理。

关键词: 蛲虫, 感染率, 幼儿园, 儿童, 健康教育, 知晓率, 高邮市