中国血吸虫病防治杂志 ›› 2022, Vol. 34 ›› Issue (5): 514-.

• 实验研究 • 上一篇    下一篇

白细胞介素9促进日本血吸虫感染小鼠肝星状细胞激活

战廷正1,马会会2,李青3,唐莉莉1,何姗姗1,唐泽丽3,夏超明2*   

  1. 1 广西医科大学寄生虫学教研室(广西 南宁530021);2 苏州大学医学部病原生物学系(江苏 苏州 215000);3 广西医科大学细胞生物学教研室
  • 出版日期:2022-11-23 发布日期:2022-11-23
  • 作者简介:战廷正,男,博士,副教授。研究方向:寄生虫感染与免疫
  • 基金资助:
    国家自然科学基金(81860358);广西壮族自治区自然科学基金(2018GXNSFAA281059)

Interleukin-9 promotes the activation of hepatic stellate cells in mice infected with Schistosoma japonicum

ZHAN Ting-zheng1, MA Hui-hui2, LI Qing3, TANG Li-li1, HE Shan⁃shan1, TANG Ze-li3, XIA Chao⁃ming2*   

  1. 1 Department of Parasitology, Guangxi Medical University, Nanning, Guangxi 530021, China; 2 Department of Pathogenic Biology, Medical College, Soochow University, Suzhou, Jiangsu 215000, China; 3 Department of Cell Biology, Guangxi Medical University, China
  • Online:2022-11-23 Published:2022-11-23

摘要: 目的 探讨小鼠感染日本血吸虫后,白细胞介素-9(interleukin-9, IL-9)在小鼠肝星状细胞活化中的作用。方法 采用肝脏原位灌注消化结合密度梯度离心法,分离日本血吸虫感染7周后小鼠原代肝星状细胞(hepatic stellate cells, HSCs)并进行体外培养。将HSCs分为PBS对照组和IL-9刺激组(浓度20 ng/mL)。分别于刺激后48、72 h收集细胞,采用免疫印迹试验检测HSCs中α平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)、Ⅰ型胶原蛋白(typeⅠcollagen, ColⅠ)和Ⅲ型胶原蛋白(type Ⅲ collagen, Col Ⅲ)表达水平。结果 经20 ng/mL IL-9刺激48 h后,IL-9刺激组HSCs中α-SMA [(0.87 ± 0.02) vs. (0.69 ± 0.01);t = 17.39,P < 0.01]、ColⅠ[(0.74 ± 0.02) vs. (0.65 ± 0.01);t = 9.56,P < 0.01]、Col Ⅲ蛋白 [(0.94 ± 0.04) vs. (0.75 ± 0.03);t = 6.15,P < 0.01]表达水平均显著高于PBS对照组;刺激72 h后,IL-9刺激组HSCs中α-SMA蛋白水平显著高于PBS对照组 [(0.76 ± 0.02) vs. (0.58 ± 0.02);t = 12.52,P < 0.01],但两组间ColⅠ[(0.68 ± 0.02) vs. (0.66 ± 0.02);t = 1.15,P > 0.05]和Col Ⅲ蛋白[(0.75 ± 0.01) vs. (0.72 ± 0.02);t = 2.22,P > 0.05]表达水平差异均无统计学意义。结论 IL-9可促进日本血吸虫感染小鼠HSCs激活。  

关键词: 日本血吸虫, 白细胞介素9, 肝星状细胞, 肝纤维化

Abstract: Objective To investigate the effects of interleukin-9 (IL-9) in the activation of hepatic stellate cells (HSCs) in mice infected with Schistosoma japonicum. Methods Primary HSCs were isolated from mice 7 weeks post-infection with S. japonicum using the in situ liver perfusion and density gradient centrifugation, and cultured in vitro. HSCs were randomly assigned to the PBS control group and IL-9 stimulation group (stimulation with 20 ng/mL IL-9). HSCs were harvested 48 h and 72 h post-stimulation, and the expression of α-smooth muscle actin (α-SMA), typeⅠcollagen (ColⅠ) and type Ⅲ collagen (Col Ⅲ) was determined in HSCs using Western blotting. Results Following stimulation with 20 ng/mL IL-9 for 48 h, the expression of α-SMA [(0.87 ± 0.02) vs. (0.69 ± 0.01); t = 17.39, P < 0.01], ColⅠ[(0.74 ± 0.02) vs. (0.65 ± 0.01); t = 9.56, P < 0.01] and Col Ⅲ [(0.94 ± 0.04) vs. (0.75 ± 0.03); t = 6.15, P < 0.01] was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group. Following stimulation with 20 ng/mL IL-9 for 72 h, the expression of α-SMA was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group [(0.76 ± 0.02) vs. (0.58 ± 0.02); t = 12.52, P < 0.01]; however, there were no significant differences between the two groups in terms of ColⅠ[(0.68 ± 0.02) vs. (0.66 ± 0.02); t = 1.15, P > 0.05] or Col Ⅲ expression [(0.75 ± 0.01) vs. (0.72 ± 0.02); t = 2.22, P > 0.05]. Conclusion IL-9 promotes the activation of HSCs in mice infected with S. japonicum.

Key words: Schistosoma japonicum, Interleukin-9, Hepatic stellate cell, Hepatic fibrosis

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