Objective To analyze the effect of ancylostomiasis control in 6 demonstration plots of parasitic disease compre? hensive control, so as to provide the evidence for formulating the prevention and control measures of ancylostomiasis in the future. Methods According to the drugs and deworming schemes, the 6 demonstration plots (counties) were divided into 4 groups, name? ly A1 (Tunchang in Hainan Province and Danling in Sichuan Province), A2 (Tongcheng in Anhui Province and Yueyang in Hunan Province), B (Guixi in Jiangxi Province), C (Rongxian in Guangxi Zhuang Autonomous Region) groups. The infection rates of hook? worm before and after the comprehensive control in the 6 counties were compared, and the costs of reducing one infected case and 1% of infection rate were calculated. Results The infection rates of hookworm in Group A1, A2, B and C were reduced by 73.36%, 93.67%, 75.95% and 59.04%, respectively. The costs of reducing one infected person were 17.33, 30.06, 11.50, 73.34, 31.28 Yuan and 27.34 Yuan in Tunchang, Danling, Tongcheng, Guixi, Rongxian and Yueyang, respectively, while those of reduc? ing 1% of the infection rate were 38 206.93, 28 577.55, 59 716.30, 295 074.91, 110 729.47 Yuan and 202 328.80 Yuan, respec? tively. Conclusions The effect of comprehensive control of ancylostomiasis in demonstration plots is remarkable. Under the same control measure, the higher the infection rates are, the less the cost will be for reducing one infected person or infection rate by 1%, and when the infection rate is below 10%, the control cost is high.