Abstract: This article presented the diagnosis and treatment of three patients from the first imported familial clustering of clonorchiasis in Shanghai Municipality, and evaluated the value of different stool examination methods in non⁃endemic areas of clonorchiasis. In February 2026, the first case of clonorchiasis in this family was confirmed, and subsequent epidemiological surveys revealed that four family members all had a history of traveling to Guangdong Province and consuming raw freshwater fish. Stool samples were collected from all four individuals and detected for Clonorchis sinensis eggs using both machine smear method and the modified Kato⁃Katz thick smear method. C. sinensis infection was detected among three individuals using the Kato⁃Katz method, with severe [20 448 eggs per gram (EPG)], moderate (2 376 EPG), and mild infections (960 EPG) identified, respectively, and no C. sinensis eggs were identified in the remaining one stool sample. In contrast, only one infection was detected with the machine smear method, resulting in a underdiagnosis rate of 66.67% (2/3). All three confirmed cases were cured following standardized therapy. This article indicates that imported familial clustering clonorchiasis cases are highly occult in non⁃endemic areas, and the machine smear method has a high risk of underdiagnosis during initial screening. A two⁃tier diagnostic model combining "machine smear method for initial screening + Kato⁃Katz method for confirmation" is recommended. 

Key words: Clonorchiasis, Familial cluster, Imported case, Diagnostic method, Underdiagnosis, Non?endemic area 

摘要： 本文报道了上海市首起输入性家庭聚集性华支睾吸虫病疫情中3例患者的诊治经过，并评价了不同粪检方法在华支睾吸虫病非流行区的应用价值。2026年2月该家庭中首例华支睾吸虫病病例被确诊，随后流行病学调查发现4名家庭成员均有广东省旅居史及淡水鱼生食史。收集4人粪便样本，采用机器涂片法与改良加藤厚涂片法（Kato-Katz法）检测粪便中华支睾吸虫虫卵。Kato-Katz法检出3例华支睾吸虫感染病例，感染程度分别为重度［每克粪便虫卵数（eggs per gram，EPG）为20 448］、中度（EPG为2 376 ）及轻度（EPG为960 ）感染；另有1人粪便样本中未检出华支睾吸虫虫卵。机器涂片法仅检出1例感染者，漏检率为66.67%（2/3）。经规范治疗，3例确诊患者均治愈。本文提示，非流行区输入性家庭聚集性华支睾吸虫病病例具有较强隐匿性，且机器涂片法初筛漏检风险较高，建议采用“机器涂片法初筛+Kato-Katz法复核”的二级诊断模式。

关键词: 华支睾吸虫病, 家庭聚集性, 输入性病例, 诊断方法, 漏诊, 非流行区