Abstract: Objective　To investigate the prevalence of tick⁃borne rickettsial infections in selected areas of Liupanshui City, Guizhou Province, 2023, so as to provide insights into the management of tick⁃borne rickettsioses in the city. Methods　Ticks were captured from the body surface of bovines and sheep in Gaoxing Village, Dashan Township, Liupanshui City, Guizhou Province during the period between April and June, 2023, and tick species were identified using morphological and molecular biological techniques. In addition, tick⁃borne Rickettsia was identified using a nested PCR assay, including spotted fever group rickettsiae (SFGR), Coxiella spp., Anaplasma spp., Ehrlichia spp., and Orientia spp., and positive amplified fragments were sequenced and aligned with known sequences accessed in the GenBank database. Results　A total of 200 ticks were collected and all tick species were identified as Rhipicephalus microplus. Nestle PCR assay combined with sequencing identified ticks carrying Candidatus Rickettsia jingxinensis (40.50%), Coxiella burnetii (1.50%), and Coxiella⁃like endosymbionts (27.00%), and Anaplasma spp., Ehrlichia spp. or Orientsia spp. was not detected. Conclusions　R. microplus carried Candidatus R. jingxinensis, C. burnetii, and Coxiella⁃like endosymbionts in selected areas of Liupanshui City, Guizhou Province. Intensified monitoring of tick⁃borne rickettsial infections is needed in livestock and humans to reduce the damages caused by rickettsioses.

Key words: Rhipicephalus microplus, Rickettsia, Candidatus Rickettsia jingxinensis, Coxiella burnetii, Coxiella?like endosymbiont, Liupanshui City

摘要： 目的　了解贵州省六盘水市部分地区蜱携带立克次体感染率，为当地蜱传立克次体病防控工作提供参考依据。方法　2023年4—6月在贵州省六盘水市盘州市大山镇高兴村捕捉牛、羊体表寄生蜱，采用形态学和分子生物学方法对蜱种进行鉴定。采用巢式PCR法检测蜱携带的立克次体，包括斑点热群立克次体、柯克斯体属、无形体属、埃立克体属和东方体属，扩增的阳性片段测序后与GenBank已知序列进行同源性比对。结果　共采集200只蜱，经鉴定均为微小扇头蜱。巢式PCR联合测序鉴定出蜱携带敬信立克次体暂定种（40.50%）、贝氏柯克斯体（1.50%）和柯克斯体共生菌（27.00%），未检测出无形体、埃立克体及东方体。结论　贵州省六盘水市部分地区微小扇头蜱携带敬信立克次体暂定种、贝氏柯克斯体和柯克斯体共生菌；当地有关部门需加强家畜和人群蜱传立克次体感染监测，减少蜱传立克次体病危害。

关键词: 微小扇头蜱, 立克次体, 敬信立克次体暂定种, 贝氏柯克斯体, 柯克斯体共生菌, 六盘水市