Abstract: Objective　To analyze the components of proteins from Echinococcus granulosus cyst fluid using the shotgun method, and to identify the active components with potential regulatory effects for immune dysregulation diseases. Methods　The   E. granulosus cyst fluid was collected aseptically from the hepatic cysts of patients with cystic echinococcosis, and characterized by liquid chromatography （LC） tandem mass spectrometry （MS/MS） following digestion with trypsin. The protein data were searched using the software MaxQuant version 1.6.1.0 and the cellular components, molecular functions, and biological processes of the identified proteins were analyzed using the Gene Ontology （GO） method. Results　The E. granulosus cyst fluid separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS⁃PAGE） had a relative molecular mass of 25 to 70 kDa. LS⁃MS/MS analysis identified 37 proteins, including 32 known proteins and 5 unknown proteins. At least 4 proteins were preliminarily found to exhibit potential regulatory effects for immune dysregulation diseases, including antigen B, glutathione⁃S⁃transferase （GST）, thioredoxin peroxidase （TPX） and malate dehydrogenase （MDH）. GO enrichment analysis showed that the identified proteins had 149 molecular functions and were involved in 341 biological processes. Conclusions 　E. granulosus cyst fluid has a variety of protein components, and four known proteins are preliminarily identified to be associated with immune dysregulation diseases.

Key words: Echinococcus granulosus, Cyst fluid, Proteomics, Shotgun, Immune dysregulation disease

摘要： 目的　利用鸟枪法分析细粒棘球蚴（Echinococcus granulosus）囊液蛋白（hydatid cyst fluid protein，HCFP）组分，寻找其中具有潜在调控免疫失调性疾病功能的活性组分。方法　无菌收集细粒棘球蚴病患者肝囊肿中的细粒棘球蚴囊液，采用鸟枪法进行液相色谱⁃串联质谱鉴定，采用MaxQuant 1.6.1.0软件进行数据库检索。利用基因本体论（Gene Ontology，GO）对已鉴定的蛋白质从细胞组分、分子功能和生物学功能三个方面进行分类。结果　经十二烷基磺酸钠⁃聚丙烯酰胺凝胶电泳（SDS⁃PAGE）分离的细粒棘球蚴囊液蛋白条带相对分子量为25～70 kDa，质谱分析共获得37个蛋白，其中已鉴定蛋白32种、未知蛋白5种。已初步发现至少有4种蛋白具有潜在调节免疫失调性疾病作用，即抗原B、谷胱甘肽S⁃转移酶、硫氧还蛋白过氧化物酶和苹果酸脱氢酶。GO富集分析显示，已鉴定蛋白具有149种分子功能，参与341种生物学过程。结论　细粒棘球蚴囊液蛋白成分复杂多样，从已知蛋白中初步筛选出4种与调节免疫失调性疾病潜在相关的蛋白。

关键词: 细粒棘球蚴, 囊液, 蛋白质组, 鸟枪法, 免疫失调性疾病