Abstract: Objective To preliminarily study the pro?angiogenic activity of Echinococcus granulosus hydatid cysts against human umbilical vein endothelial cells in vitro and the transcriptional level of potential pro?angiogenic factors. Methods The hydatid cysts and protoscolex derived from experimentally infected mice were collected and cultured in vitro， then the human umbilical vein endothelial cells were stimulated by the supernatant and cyst fluid respectively， and the angiogenesis was observed and analyzed through a microscope and the angiogenesis mode of the software NIH Image J. Meanwhile， the mouse homologous proteins of matrix metalloproteinase?9 （MMP?9） and high mobility group box B1 （HMGB1） were identified in E. granulosus genome through sequence alignment， and their transcriptional levels in the cyst wall and protoscolex were analyzed. Results The culture supernatant of hydatid cysts significantly promoted human umbilical vein endothelial cells into tubes （F = 73.03， P < 0.001）， the transcriptions of MMP?9 and HMGB1 were detected in the cyst wall and protoscolex， and the transcriptional level of MMP?9 was higher in protoscolex （t = -11.65， P < 0.001），while the level of HMGB1 was higher in hydatid cysts （t = 6.43， P = 0.003）. Conclusion Some parasite?derived pro?angiogenic molecules may exist in the supernatant of E. granulosus hydatid cysts， while further researches are required into their exact mechanisms.

Key words: Echinococcus granulosus； Angiogenesis； Matrix metalloproteinase?9 （MMP?9）； High mobility group box B1 （HMGB1）； In vitro

摘要： 目的 分析细粒棘球蚴体外对人脐静脉内皮细胞血管生成能力的影响及其促血管生成因子转录水平，初步探讨细粒棘球蚴的促血管生成作用。方法 将继发感染小鼠体内的细粒棘球蚴体外培养后的上清作用于体外培养的人脐静脉内皮细胞，观察其成管现象并用NIH Image J软件的Angiogenesis模块进行分析。同时，在细粒棘球蚴基因组中寻找同宿主（小鼠）的基质金属蛋白酶9（Matrix metalloproteinase?9，MMP?9）和高迁移率族蛋白B1（High mobility group box B1，HMGB1）的同源蛋白，并对其转录水平进行分析。结果 鼠源细粒棘球蚴囊培养上清能显著促进人脐静脉内皮细胞的成管（F = 73.03，P < 0.001），在细粒棘球蚴囊壁和原头节中存在小鼠MMP?9和HMGB1同源蛋白的表达，且MMP?9在原头节中的转录水平高于棘球蚴囊（t = -11.65，P < 0.001），而HMGB1在棘球蚴囊中的转录水平高于原头节（t = 6.43，P = 0.003）。结论 细粒棘球蚴囊培养上清可能含有促进血管生成的虫源分子，但其确切机制还有待进一步研究。

关键词: 细粒棘球蚴；血管生成；基质金属蛋白酶?9；高迁移率族蛋白B1；体外试验