Chin J Schisto Control ›› 2017, Vol. 29 ›› Issue (2): 228-230.
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CHEN Chuang1*| CHEN Xing-wang1| DENG Jian-zhong2| LI Tiao-ying1
Online:
Published:
陈闯1*|陈兴旺1|邓建中2|李调英1
作者简介:
基金资助:
Abstract: Objective To evaluate the ELISA kit for detection of IgG antibodies against Taenia solium cysticercus in humans, so as to provide a reference for its application in clinical practice. Methods The sera collected from the patients with neurocysticercosis, echinococcosis, taeniasis and healthy people, respectively, were checked by ELISA for specific IgG antibodies against T. solium cysticercus, as described in the instruction of the kit. Results Of the 30 patients with neurocysticercosis at active stage, 28 showed seropositive reaction, indicating a sensitivity of 93.33%. There were no positive reactions in the 100 healthy people. Two of the 42 persons with taeniasis were seropositive with an infection rate of 4.76%. Forty?one of the 60 persons with echinococcosis showed positive reactions, suggesting that the cross reaction rate of the kit with echinococcosis was 68.33%. Conclusions The assessed ELISA kit has a reasonably high sensitivity but a poor specificity, and the cross reaction rate with echinococcosis is very high. It is urgent to improve the sensitivity and specificity of the diagnostic kit for detection of T. solium cysticercosis in China.
Key words: Cysticercosis; ELISA; IgG antibody; Sensitivity; Specificity; Kit
摘要: 目的 评价囊虫IgG抗体检测试剂盒的诊断效果,为临床应用提供参考。方法 按照囊虫IgG抗体检测试剂盒说明书,采用ELISA法检测囊虫病、包虫病、带绦虫病及健康人血清。结果 在30例活动期脑囊虫病病人中,28例呈囊虫IgG抗体阳性,敏感性为93.33%。100份健康人血清均无阳性反应。在42例带绦虫感染者,2例囊虫抗体阳性,阳性率为4.76%。在60份包虫病血清中,阳性41份,该试剂盒与包虫病的交叉反应率为68.33%。结论 所评价试剂盒敏感性较好,但特异性差,与包虫病的交叉反应率高。以上提示国内囊虫病免疫学诊断试剂的质量有待提高。
关键词: 囊虫病;ELISA;IgG抗体;敏感性;特异性;试剂盒
CLC Number:
R532.3
CHEN Chuang, CHEN Xin-Wang, DENG Jian-Zhong, LI Tiao-Yang. Evaluation of ELISA kit for detection of serum specific IgG antibodies against Taenia solium in diagnosis of human cysticercosis[J]. Chin J Schisto Control, 2017, 29(2): 228-230.
陈闯, 陈兴旺, 邓建中, 李调英. 人体囊虫病诊断试剂盒的效果评价[J]. 中国血吸虫病防治杂志, 2017, 29(2): 228-230.
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