Chin J Schisto Control ›› 2014, Vol. 26 ›› Issue (4): 431-.

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Effect of Parasep? feces centrifuge tube method on detecting schistosome eggs

MA Nian1 |ZHANG Hua-ming1 |LIU Xiong1 |XIAO Chuan-yun1 |WEN Xiao-hong1 |LI Xia1 |DONG Li-chun1 |CUI Cai-xia1 |TU Zu-wu2*   

  1. 1 Jiangling Institute of Schistosomiasis Prevention and control|Hubei Province|Jiangling 434100|China;2 Institute of Schisto? somiasis Control|Hubei Provincial Center for Disease Control and Prevention|China
  • Online:2014-08-15 Published:2014-08-12
  • Contact: TU Zu?wu

Parasep?粪便离心管法检测血吸虫虫卵效果

马念1|张华明1|刘雄1|肖传云1|文晓红1|李霞1|董黎春1|崔彩霞1|涂祖武2*   

  1. 1湖北省江陵县血吸虫病预防控制所(江陵434100); 2 湖北省疾病预防控制中心血吸虫病防治研究所
  • 通讯作者: 涂祖武
  • 作者简介:马念| 女| 检验技师。研究方向: 血吸虫病诊断与防治
  • 基金资助:
    湖北省卫生厅血吸虫病防治科研项目 (XF2010?29)

Abstract: Objective Objective To evaluate the effect of the Parasep? feces centrifuge tube method on detecting schistosome eggs. Methods Methods A total of 803 residents aged from 6-65 years were selected in 2 schistosomiasis endemic villages,Jiangling Coun? ty,Hubei Province,and their stool samples were collected and detected parallelly by the Kato?Katz technique,nylon silk egg hatching method,and Parasep? feces centrifuge tube method at the same time. Results Results Among the 803 people,15 cases were found of schistosome egg positive,and the positive rate was 1.87%. The positive rates of the Kato?Katz technique,nylon silk egg hatching method,and Parasep? feces centrifuge tube method were 0.75%,1.49% and 1.12%,respectively. The schistosome eggs got with the Parasep? feces centrifuge tube method were clear and easy to identify. Conclusion Conclusion In low endemic areas of schistosomiasis,the Parasep? feces centrifuge tube method can be used as schistosomiasis japonica etiology diagnosis method.

Key words: Schistosomiasis japonica; Parasep? fecal centrifuge tube method; Diagnosis

摘要: 目的 目的 探讨Parasep?粪便离心管法在血吸虫病病原学检测中的应用效果。方法 方法 选择湖北省江陵县2个血吸 虫病流行村6~65岁居民为研究对象, 采用改良加藤厚涂片法 (Kato?Katz法)、 尼龙绢集卵孵化法和Parasep?粪便离心管 法进行病原学平行检测, 对检测结果进行比较分析。结果 结果 2个村共检测803人, 共查出血吸虫虫卵阳性15例, 平均粪 检阳性率为1.87%。其中Kato?Katz法、 尼龙绢集卵孵化法和Parasep?粪便离心管法粪检阳性率分别为0.75%、 1.49%和 1.12%, Parasep?粪便离心管法的检出率是Kato?Katz法的1.49倍。2个村Kato?Katz法的漏检率最高, 分别为57.14%和 62.50%, 尼龙绢集卵孵化法最低, 分别为14.29%和25.00%。Parasep?粪便离心管法可使显微镜下虫卵形态不变, 卵内毛 蚴结构清晰, 容易辨认。结论 结论 在血吸虫病低度流行区, Parasep?粪便离心管法可作为日本血吸虫病病原学诊断的方法。

关键词: 日本血吸虫病; Parasep®粪便离心管法; 诊断

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