Abstract: Objective To explore the effect of Pulsatilla chinensis（Bunge）Regel saponins（PRS）against juvenile and adult Schistosoma japonicum and to compare its efficacy with praziquantel（PZQ）in vitro. Methods 3 h，7 d，14 d schistosomula and 42 d adult schistosomes were incubated with 0，1，5，10，20 and 30 μg/ml PRS for 4，24，48 and 72 hours，then the states of them were observed. The changes of the surface of S. japonicum incubated with 30 μg/ml PRS and PZQ within 4 hours were ob? served by a scanning electron microscope. Results The sensitivity of 3 h，7 d，14 d schistosomula and adults of S. japonicum to 1，5，10，20，30 μg/ml PRS displayed a time and dose dependence. All the worms died in 30 μg/ml PRS after 4 hours. The dead worm body appeared a gray?white color accompanied with their altered morphogenesis and opaque body. The tegumental surface of adults with different degrees of damages was observed by the electron microscope within 4 hours affected by PRS in vitro. Conclu? sions The effects of PRS against S. japonicum in different developmental stages in vitro show that PRS may eventually have a ther? apeutic potential in the treatment or prevention of S. japonicum infection and is expected to become a new anti?schistosome drug.

Key words: chistosoma japonicum；Pulsatilla chinensis（Bunge）Regel saponins；Adult worm；Juvenile worm；In vitro effi? cacy

摘要： 目的 目的 探讨白头翁总皂苷 （Pulsatilla chinensis（Bunge）Regel saponins， PRS） 对不同发育阶段的日本血吸虫的体外 杀灭效果。方法 方法 日本血吸虫3 h和7、 14 d童虫及成虫分别用0、 1、 5、 10、 20、 30 μg/ml PRS及30 μg/ml吡喹酮 （PZQ） 孵育 4、 24、 48、 72 h后， 观察虫体变化及状态； 用扫描电镜观察30 μg/ml PRS及PZQ孵育4 h内的日本血吸虫成虫的体表变化。 结果 结果 PRS对日本血吸虫3 h和7、 14 d童虫及成虫的体外杀灭效果呈时间和剂量依赖性。经30 μg/ml PRS作用4 h后， 日 本血吸虫童虫及成虫全部死亡； 死亡虫体颜色发暗， 形态发生改变且虫体不透明。扫描电镜观察发现， PRS作用后的日本 血吸虫成虫体表有不同程度损伤。结论 结论 传统中草药PRS可能对日本血吸虫感染有一定的治疗和预防作用， 有希望成为 新的抗血吸虫药物

关键词: 日本血吸虫； 白头翁总皂苷； 成虫； 幼虫； 体外作用