Abstract: Objective To evaluate the effect of a novel plant molluscicide，4% “Luo ?wei” （Tea ? seed distilled saponins， TDS）against Oncomelania hupensis snails in plateau mountain areas in Yunnan Province. Methods The immersing and spraying experiments were carried out in the ditches and grassland of Xiaolian Administrative Village in Heqing County，Yunnan Province， to assess the molluscicidal effect of 4% TDS comparing with 50% wettable powder of niclosamide ethanolamine salt（WPN）in dif? ferent environments and time. Results After immersion for 24，48 h and 72 h，the snail death rates were 70.67%，87.33% and 98.67% in the TDS group，whereas being 77.33%，96.67% and 100.00% in the WPN group，respectively. The differences of the death rates between the two groups 24 h and 72 h after immersing were not statistically significant（χ2 24 h =1.73，χ2 72 h =2.01， both P values > 0.05） . Seven days after the immersing experiments，the occurrence rate of frames with living snails and the death rate of snails were 20.00% and 93.03% in the TDS group，while those were 13.33% and 95.76% in the WPN group，and there were no significant differences of the 2 indexes between the 2 groups（χ2 Occurrence rate =2.27，χ2 Dea th rate =0.94，all P values > 0.05） . After spraying for 1，3，7 d and 15 d，in both groups，the occurrence rates of frames with living snails and the average densities of living snails gradually declined，while the death rates of snails gradually increased with the extension of time. There were no sta? tistically significant differences of the above 3 indexes between the 2 groups（all P values > 0.05） . Fifteen days after the spray? ing experiment，the occurrence rate of frames with living snails and the adjusted death rate of snails were15.00% and 87.39％ in the TDS group and those were16.67% and 89.32% in the WPN group，respectively. Conclusion The molluscicidal effect of TDS is satisfying in plateau mountain areas，and the molluscicide is worthy of further extension and application.

Key words: Oncomelania hupensis；Tea seed distilled saponin（TDS）；Niclosamide ethanolamine salt（WPN）；Mollusci? cidal effect；Plateau mountain area

摘要： 目的 目的 评价植物杀螺剂4%螺威粉剂 （TDS） 在云南高原山区的灭螺效果。方法 方法 在云南省鹤庆县选择沟渠和草 地， 采用2.5 g/m3 和5 g/m2 TDS开展浸杀和喷洒试验， 同时以 50%氯硝柳胺乙醇胺盐可湿性粉剂 （WPN） 作为对照， 比较两组 不同环境、 时间的杀螺效果。结果 结果 浸杀后24、 48、 72 h， TDS组钉螺死亡率分别为70.67%、 87.33%、 98.67%， WPN组分别为 77.33%、 96.67%、 100.00%， 浸杀后24、 72 h钉螺死亡率差异均无统计学意义 （χ2 24 h =1.73，χ2 72 h =2.01，P均＞0.05）； 浸杀后 7 d， TDS组活螺框出现率和钉螺死亡率分别为20.00%和93.08％， WPN组分别为13.33%和95.76％， 两组比较差异均无统 计学意义 （ χ2 出现率 = 2.27，χ2 死亡率 = 0.94，P均＞0.05）。喷洒灭螺后1、 3、 7、 15 d， 随时间延长两组活螺框出现率和活螺平均 密度均逐渐下降， 钉螺死亡率逐渐增高， 但各指标差异均无统计学意义 （P均＞0.05）； 喷洒后15 d， TDS组活螺框出现率和 校正钉螺死亡率分别为15.00%和87.39％， WPN组分别为16.67%和89.32％。结论 结论 TDS在云南高原山区现场灭螺效果良 好， 值得进一步推广应用。

关键词: 钉螺； 4%螺威粉剂； 氯硝柳胺乙醇胺盐； 灭螺效果； 高原山区