Abstract:

Objective To study the detection effects of ELISA for the infections of Cysticercus，Echinococcosus，Paragoni? mus westermani and Sparganum mansoni in order to improve diagnosis. Methods The patients who admitted to Jiangsu Institute of Parasitic Diseases from 2008 to 2001 were selected as the subjects and their sera were detected for IgG against Cysticercus，Echi? nococcosus，and Paragonimus westermani from January 2008 to March 2011，and IgG against Sparganum mansoni since June， 2011 by ELISA. Results A total of 282 cases were detected，and 88 of them were found one or two positive results，and the total positive rate was 31.2%. There were 39，2，21，and 3 cases of positive IgG of Cysticercus，Echinococcosus，Paragonimus wester? mani and Sparganum mansoni，respectively. There were 15 cases of positive IgG to both Cysticercus and Echinococcosus；there were 5 cases of positive IgG to both Cysticercus and Paragonimus westermani；there were 3 cases of positive IgG to both Echinococ? cosus and Paragonimus westermani. The cross reactions occurred in 23 serum samples among 88 positive samples with a cross reac? tion rate of 26.1%. Conclusion There still exist a certain number of patients with parasitic diseases，and the cross reaction may occur in the detection process of IgG to Cysticercus， Echinococcosus，Paragonimus westermani and Sparganum mansoni by ELISA.

Key words: Parasitic disease；IgG；ELISA； Cysticercus；Echinococcosus； Paragonimus westermani； Sparganum mansoni

摘要：

目的 观察酶联免疫吸附试验 （ELISA） 检测猪囊尾蚴、 细粒棘球蚴、 卫氏并殖吸虫和曼氏裂头蚴感染的效果。方法 以2008-2011年就诊于江苏省寄生虫病防治研究所的患者为检测对象， 采用ELISA法检测血清抗体。每份血清均检测囊尾蚴、 棘球蚴和卫氏并殖吸虫IgG抗体， 2011年6月起增加检测曼氏裂头蚴IgG抗体。结果 共检测282例患者， 其中 IgG抗体检测1项或2项同时阳性者88例， 总阳性率为31.2%； 囊尾蚴、 棘球蚴、 卫氏并殖吸虫、 曼氏裂头蚴IgG抗体1项阳性者分别为39、 2、 21、 3例； 囊尾蚴和棘球蚴IgG抗体2项同时阳性者15例， 囊尾蚴和卫氏并殖吸虫抗体同时阳性者5例， 棘球蚴和卫氏并殖吸虫抗体同时阳性者3例， 88例阳性标本中交叉反应者23例， 交叉反应率为26.1%。结论 目前仍存在一定数量的寄生虫感染者。ELISA检测抗寄生虫IgG抗体存在交叉反应。

关键词: 寄生虫病； IgG抗体； 酶联免疫吸附试验； 猪囊尾蚴； 细粒棘球蚴； 卫氏并殖吸虫； 曼氏裂头蚴