Abstract:

Dechlorinated water（100 ml，30℃）was put into a plate（diameter 15 cm），and 1% niclosamide spreading oil 5 μl was added，then a ring of Schistosoma japonicum cercariae were picked up to the plate. The time of killing all the cercariae was observed at three time points（immediately，24，48 h），and the dechlorinated water was used as control. The results showed that schistosome cercariae were all killed in three minutes by 1% niclosamide spreading oil at the three time points. The cercaria?kill? ing effects of each time point were not significantly different（F = 0.062， P > 0.05） . The cercariae were alive in the control in 48 h.

Key words: Niclosamide spreading oil； Schistosome； Cercaria

摘要：

取100 ml 30 ℃去氯水加入直径15 cm的平皿中， 然后加入1%氯硝柳胺展膜油剂5 μl， 再用接种环挑取一环人工逸出的尾蚴于平皿中央， 立即在解剖镜下观察尾蚴全部死亡所需要时间， 并对加入1%氯硝柳胺展膜油剂放置0、 24、 48 h后的杀蚴效果进行观察。同时用去氯水作对照。 放置3个不同时间段的1%氯硝柳胺展膜油剂均能在3 min内将尾蚴全部杀死， 灭蚴效果差异无统计学意义 （F = 0.062，P＞ 0.05）， 对照组尾蚴48 h仍有活性。

关键词: 氯硝柳胺油扩散剂； 血吸虫； 尾蚴