Abstract: Objective Objective To comprehensively evaluate the effects of indirect hemagglutination test（IHA），enzyme?linked im? munosorbent assay（ELISA），and dipstick dye method（DDIA）in the diagnosis of schistosomiasis japonica at different preva? lence by using Meta?analysis. Methods Methods Through the literature review according to the inclusion and exclusion criteria，a data? base was established，and by using Meta?disc and R software，the Meta?analysis was performed including the threshold test，het? erogeneity test，weighted by the quantitative effect of merger，SROC curve fitting，etc. Results Results A total of 60 papers were in? cluded in the final analysis. The sensitivities of IHA were 0.84，0.76 and 0.94 in heavy，medium and low endemic areas，and specificities were 0.73，0.64 and 0.73 respectively；the sensitivities of ELISA were 0.88，0.80 and 0.93 in heavy，medium and low endemic areas，and the specificities were 0.59，0.59 and 0.62 respectively；the sensitivities of DDIA were 0.93，0.81 and 0.93 in the heavy，medium and low endemic areas，and specificities were 0.66，0.69 and 0.59 respectively. The weighted sensi? tivities of IHA，ELISA and DDIA were 0.83，0.87 and 0.90 respectively；the weighted specificities were 0.69，0.60 and 0.62 re? spectively. The areas under the curve of SROC were 0.89，0.96 and 0.92 in IHA，ELISA and DDIA respectively. Conclusions Conclusions In different prevalence，the effectiveness of different methods for serological diagnosis of schistosomiasis is different. The sensi? tivity and specificity of all diagnostic methods of schistosomiasis need to further improve.

Key words: Schistosomiasis japonica, Indirect hemagglutination test（IHA）, Enzyme? linked immunosorbent assay（ELI? SA）, Dipstick dye method（DDIA）, Sensitivity, Specificity, Diagnostic effectiveness, Meta?analysis

摘要： 目的 目的 通过Meta分析， 综合评价不同流行程度下间接血凝实验 （IHA）、 酶联免疫吸附测定 （ELISA） 和胶体染料 试纸条法 （DDIA） 的日本血吸虫病诊断效果。方法 方法 通过文献检索， 按照纳入和排除标准建立数据库， 采用Meta?disc和 R软件进行阈值检验、 异质性检验、 效应量加权定量合并及SROC曲线拟合等Meta分析。结果 结果 共有60篇文献符合纳入 标准。IHA在重、 中度及低度流行区的灵敏度分别为0.84、 0.76和0.94， 特异度分别为0.73、 0.64和0.73； ELISA在重、 中度 及低度流行区的灵敏度分别为0.88、 0.80和0.93， 特异度分别为0.59、 0.59和0.62； DDIA在重、 中度及低度流行区的灵敏 度分别为0.93、 0.81和0.93， 特异度分别0.66、 0.69和0.59。IHA、 ELISA与DDIA的加权合并灵敏度分别为0.83、 0.87和 0.90； 加权合并特异度为0.69、 0.60和0.62。IHA、 ELISA和DDIA的总体SROC曲线下面积分别为0.89、 0.96和0.92。结 结 论 论 不同流行程度下， 血吸虫病血清学诊断方法的检验效能存在一定差异。血吸虫病诊断试剂的灵敏度与特异度均需 进一步提高

关键词: 日本血吸虫病, 间接血凝实验 （IHA）, 酶联免疫吸附测定 （ELISA）, 胶体染料试纸条法 （DDIA）, 灵敏度, 特异 度, 检验效能, Meta分析