关键词: 棘球绦虫； IgG抗体； 酶联免疫吸附试验； 试剂盒； 交叉反应

Abstract:

Objective To compare the effects of two ELISA kits on IgG antibody detection of human Echinococcus granulo? sus. Methods A Total of 134 sera of patients with echinococcosis，paragonimiasis westermani，clonorchiasis sinensis，schistoso? miasis japonica，and cysticercosis cellulosae，and normal persons were detected by two IgG ELISA kits produced by different com? panies. Furthermore，the specificity，sensitivity and cross reactivity were counted and analyzed statistically. Results The sensi? tivity and specificity were extremely high of the two kits as 100.00%. The cross?reactivity rates were 25.00%（paragonimiasis wes? termani），26.09%（clonorchiasis sinensis），10.00%（schistosomiasis japonica），and 87.5%（cysticercosis），respectively，by us? ing the kit produced by the Combined Company in Shenzhen；the cross?reactivity rates were 5.00%（paragonimiasis westermani）， 13.04%（clonorchiasis sinensis），20.00%（schistosomiasis japonica），and 93.75%（cysticercosis）respectively，by using the kit produced by Haitai Company in Zhuhai. In addition，there was a significant difference of Paragonimus westermani detection（P < 0.05），but the rests had no statistically significant differences（all P > 0.05）between the two kits. Conclusion Both ELISA kits on IgG antibody detection of human Echinococcus granulosus have the advantages of a high sensitivity，specificity，convenience and high?speed. However，it is also in urgent need to further solve the cross?reactivity of Echinococcus granulosus with other para? sites，in order to improve the accuracy of early diagnosis.

Key words: Echinococcus granulosus； IgG antibody； Enzyme?linked immunosorbent assay（ELISA）； Kit；Cross?reaction