关键词: 日本血吸虫病； 大肠癌； 血小板衍生内皮细胞生长因子； 血管内皮生长因子

Abstract: Objective To investigate the differences of mRNA quantitation and protein expression of vascular growth factors including platelet?derived endothelial cell growth factor（PD?ECGF）and vascular endothelial growth factor（VEGF）in intestinal tissues in colorectal carcinoma patients with and without schistosomiasis. Methods Thirty colorectal carcinoma patients with schistosomiasis and 30 colorectal carcinoma patients without schistosomiasis were included in this study. The mRNA quantitation and protein expression of PD?ECGF and VEGF in the normal tissue，peri?carcinoma tissue as well as carcinoma tissue obtained from surgical specimens were detected by qRT?PCR and Western blot. Results The mRNA relative quantitations of PD?ECGF in normal tissue，peri ?carcinoma tissue and carcinoma tissue in the colorectal carcinoma patients with schistosomiasis were 1.726， 1.766 and 2.729 times to those in the colorectal carcinoma patients without schistosomiasis，respectively. The corresponding ones of VEGF were 2.138，1.831 and 3.376 times，respectively. The protein expression levels of PD?ECGF and VEGF in normal tis? sue，peri?carcinoma tissue and carcinoma tissue were higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis. Conclusions The expressions of vascular growth factors including PD?EC? GF and VEGF are higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis. Therefore，schistosomiasis may be one of the risk factors of colorectal cancer.

Key words: Schistosomiasis japonica；Colorectal carcinoma；Platelet ?derived endothelial cell growth factor（PD?ECGF）； Vascular endothelial growth factor（VEGF）