关键词: 日本血吸虫病, 吡喹酮, 抗体转归, 免疫学检测, Meta分析

Abstract: Objective　To evaluate the conversion of serum antibodies against Schistosoma japonicum in humans and livestock detected by immunological tests following treatment with praziquantel. Methods　The studies pertaining to serological tests of schistosomiasis japonica published from 1991 to 2020 were retrieved in electronic databases, including Chinese National Knowledge Infrastructure, WanFang Data, PubMed and ScienceDirect. Data were extracted from included studies. The publication bias was assessed with funnel plots using the software RevMan version 5.3, and the conversion of antibodies against S. japonicum was evaluated through meta?analysis. Results　A total of 40 publications were included in the final meta?analysis, consisting of 33 Chinese publications and 7 English publications, and all immunological tests were performed with indirect hemagglutination test (IHA) and enzyme?linked immunosorbent assay (ELISA). Pooled analysis showed that the negative rates of serum anti?S. japonicum antibody were 45.36% [95% confidential interval (CI): (43.96%, 46.76%)] and 20.83% [95% CI: (19.69%, 21.97%)] detected by ELISA and IHA within 6 months post praziquantel treatment, 62.95% [95% CI: (61.59%, 64.31%)] and 55.61% [95% CI: (54.21%, 57.01%)] within 6 to 12 months after treatment and 85.92% [95% CI: (84.94%, 86.90%)] and 86.90% [95% CI: (85.95%, 87.85%)] over 12 months after treatment, respectively. Conclusions　The negative rate of the serum anti?S. japonicum antibody by IHA and ELISA increased with the time of post?treatment with praziquantel. The overall negative rates of anti?S. japonicum antibody detected by IHA and ELISA are low within 12 months post praziquantel treatment. However, a high negative rate of anti?S. japonicum antibody is detected if there is no new contact with infested water after 12 months of praziquantel treatment.

Key words: Schistosomiasis japonica, Praziquantel, Antibody conversion, Immunological test, Meta?analysis