关键词: 血吸虫, 毛蚴, 尼龙绢集卵孵化实验, 自动摄影术

Abstract:

Objective Objective To explore the value of automatic photography in the observation of results of Schistosoma japoni? cum miracidium hatching experiments. Methods Methods Some fresh S. japonicum eggs were added into cow feces，and the samples of feces were divided into a low infested experimental group and a high infested group（40 samples each group） . In addition，there was a negative control group with 40 samples of cow feces without S. japonicum eggs. The conventional nylon bag S. japonicum miracidium hatching experiments were performed. The process was observed with the method of flashlight and magnifying glass combined with automatic video（automatic photography method），and，at the same time，with the naked eye observation meth? od. The results were compared. Results Results In the low infested group，the miracidium positive detection rates were 57.5% and 85.0% by the naked eye observation method and automatic photography method，respectively（χ2 = 11.723，P > 0.05） . In the high infested group，the positive detection rates were 97.5% and 100% by the naked eye observation method and automatic pho? tography method，respectively（χ2 = 1.253，P < 0.05） . In the two infested groups，the average positive detection rates were 77.5% and 92.5% by the naked eye observation method and automatic photography method，respectively（ χ2 = 6.894，P > 0.05） . Conclusion Conclusion The automatic photography can effectively improve the positive detection rate in the S. japonicum miracidi? um hatching experiments.

Key words: Schistosoma japonicum, Miracidium, Hatching experiment with the nylon bag, Automatic photography