云南省钉螺滇川亚种线粒体遗传分化与空间变量相关性

中国血吸虫病防治杂志（中英文） ›› 2026, Vol. 38 ›› Issue (1): 54-59.

云南省钉螺滇川亚种线粒体遗传分化与空间变量相关性

张园园1△，宋静2, 3△，郝瑜婉4，杨早改5，史欣平5，宁斯奇1，王洪琼2，
杜春红2，周济华2，张宗亚2，李凯6，李石柱4*，董毅2*

1 大理大学公共卫生学院（云南大理 671000）；2 云南省地方病防治所，云南省自然疫源性疾病防控技术重点实验室（云南昆明 650500）；3 云南省公共卫生与生物安全重点实验室（云南昆明 650500）；4 中国疾病预防控制中心寄生虫病预防控制所（上海 200025）；5 昆明医科大学；6 云南省水文水资源局大理分局

出版日期:2026-02-25 发布日期:2026-04-10
通讯作者: 李石柱 lisz@chinacdc.cn，董毅 dydali@sina.com
作者简介:张园园，女，硕士研究生。研究方向：血吸虫病流行病学与控制技术宋静，女，硕士，副主任医师。研究方向：血吸虫病防治
基金资助:
国家卫生健康委寄生虫病原与媒介生物学重点实验室2023年开放课题（NHCKFKT2023⁃08)；云南省公共卫生与生物安全重点实验室开放课题（KLPHB⁃2023⁃04）；大理州科技计划项目（20242901A020013）；云南省地方病防治所青年科技人才培养项目（YIEDC⁃T202504）

Correlation of mitochondrial genetic differentiation and spatial variables of Oncomelania hupensis robertsoni in Yunnan Province

ZHANG Yuanyuan1△, SONG Jing2, 3△, HAO Yuwan4, YANG Zaogai5, SHI Xinping5, NING Siqi1, WANG Hongqiong2, DU Chunhong2, ZHOU Jihua2, ZHANG Zongya2, LI Kai6, LI Shizhu4*, DONG Yi2*

1 School of Public Health, Dali University, Dali, Yunnan 671000, China; 2 Yunnan Institute of Endemic Disease Control and Prevention, Yunnan Key Laboratory of Natural Focus Disease Control Technology, Kunming, Yunnan 650500, China; 3 Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming, Yunnan 650500, China; 4 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China; 5 Kunming Medical University, China; 6 Dali Branch of Yunnan Provincial Hydrological and Water Resources Bureau, China

Online:2026-02-25 Published:2026-04-10

摘要/Abstract

摘要： 目的　分析云南省钉螺滇川亚种遗传分化的潜在空间影响因素。方法　于云南省血吸虫病流行区选取13个行政村作为采样点进行钉螺采样，每点采集≥ 200只钉螺并记录采样点经纬度、海拔等空间变量数据。以爬行法结合逸蚴法于每个采样点筛选出30只活力良好的血吸虫感染阴性钉螺。提取钉螺线粒体基因，经PCR扩增、产物纯化及测序后，采用DNAstar软件和NCBI数据库对样本序列进行拼接、编辑，获得各采样点钉螺线粒体完整序列。计算各采样点钉螺滇川亚种线粒体遗传距离矩阵。采用ArcGIS 10.2软件标注各采样点地理坐标，计算各采样点间直线地理距离；采用Excel软件分别计算各采样点间海拔、经度差值和纬度差值。采用Mantel检验分析云南省13个采样点钉螺滇川亚种线粒体遗传距离矩阵与各空间变量矩阵的相关性。结果　云南省13个采样点中，南涧彝族自治县安定村和弥渡县蔡庄村的钉螺种群线粒体遗传距离最大，为26.244 2。古城区东元村与楚雄县苍岭村地理距离最远，为272.64 km。南涧彝族自治县安定村与古城区东元村海拔差值最大，为1 086.10 m。洱源县前甸村与楚雄县苍岭村经度差值最大，为1.86°；南涧彝族自治县乐秋村与古城区东元村纬度差值最大，为1.81°。云南省13个钉螺滇川亚种种群线粒体遗传距离与海拔呈正相关（r = 0.542 8，P < 0.001），与地理距离（r = 0.093 4）、经度（r = -0.199 5）和纬度（r = 0.205 7）相关性均无统计学意义（P均> 0.05）。结论　海拔可能是影响云南省钉螺滇川亚种遗传分化的潜在空间因素。

关键词: 湖北钉螺滇川亚种, 遗传分化, 海拔, 经度, 纬度, 云南省

Abstract: Objective　To analyze the potential spatial factors affecting the genetic differentiation of Oncomelania hupensis robertsoni in Yunnan Province. Methods　A total of 13 administrative villages were selected from schistosomiasis⁃endemic areas of Yunnan Province as O. hupensis snail sampling sites. At least 200 snails were collected in each site, and the spatial variable data of each site were recorded, including longitude, latitude and altitude. Thirty active and Schistosoma japonicum uninfected O. hupensis snails were selected from each sampling site by means of the crawling method and the cercarial shedding method. Genomic DNA was extracted from O. hupensis snails. Following PCR amplification, purification of PCR amplification products and sequencing, the gene sequences of O. hupensis snail samples were spliced and edited using the DNAstar software and the NCBI database to yield the complete mitochondrial sequences of O. hupensis snails at each sampling site, and the mitochondrial genetic distance matrix of O. hupensis robertsoni was calculated at each sampling site. The geographical coordinates of each sampling site were marked using the software ArcGIS 10.2, and the straight⁃line geographical distance between each sampling site was calculated. The altitude difference, longitude difference and latitude difference between each sampling site were calculated using the Excel software, and the correlation between the mitochondrial genetic distance matrix of O. hupensis robertsoni and each spatial variable matrix was examined by using the Mantel test at 13 sampling sites in Yunnan Province. Results　Among the 13 O. hupensis snail sampling sites in Yunnan Province, the largest mitochondrial genetic distance of O. hupensis robertsoni snail populations was seen between Anding Village, Nanjian Yi Autonomous County and Caizhuang Village, Midu County (26.244 2), and the largest geographical distance was seen between Dongyuan Village, Gucheng District and Cangling Village, Chuxiong County (272.64 km). The highest altitude difference was seen between Anding Village, Nanjian Yi Autonomous County and Dongyuan Village, Gucheng District (1 086.10 m), and the largest longitude difference was found between Qiandian Village, Eryuan County and Cangling Village, Chuxiong County (1.86°), while the largest latitude difference was measured between Leqiu Village, Nanjian Yi Autonomous County and Dongyuan Village, Gucheng District (1.81°). In addition, the mitochondrial genetic distance of O. hupensis robertsoni snail populations was positively correlated with altitude at 13 snail sampling sites in Yunnan Province (r = 0.542 8, P < 0.001), and showed no significant correlations with geographical distance (r = 0.093 4, P > 0.05), longitude (r = −0.199 5, P > 0.05) or latitude (r = 0.205 7, P > 0.05). Conclusion　Altitude may be a potential spatial factor affecting the genetic differentiation of O. hupensis robertsoni in Yunnan Province.

Key words: Oncomelania hupensis robertsoni, Genetic differentiation, Altitude, Longitude, Latitude, Yunnan Province

中图分类号:

R383.24

张园园, 宋静, 郝瑜婉, 杨早改, 史欣平, 宁斯奇, 王洪琼, 杜春红, 周济华, 张宗亚, 李凯, 李石柱, 董毅. 云南省钉螺滇川亚种线粒体遗传分化与空间变量相关性[J]. 中国血吸虫病防治杂志（中英文）, 2026, 38(1): 54-59.

ZHANG Yuanyuan, SONG Jing, HAO Yuwan, YANG Zaogai, SHI Xinping, NING Siqi, WANG Hongqiong, DU Chunhong, ZHOU Jihua, ZHANG Zongya, LI Kai, LI Shizhu, DONG Yi. Correlation of mitochondrial genetic differentiation and spatial variables of Oncomelania hupensis robertsoni in Yunnan Province[J]. Chinese Journal of Schistosomiasis Control, 2026, 38(1): 54-59.

[1]	唐烨榕, 周红宁, 吴超, 魏春, 赵晓涛, 王雪菲, 郭小连, 姜进勇. 2023年云南省登革热省内跨县（市区）输入性病例流行病学特征[J]. 中国血吸虫病防治杂志（中英文）, 2025, 37(5): 524-529.
[2]	刘剑峰, 王文雅, 张米禛, 周长海, 刘柳, 赵陆源, 李科荣, 张冬琦, 刘榆华, 刘宏坤, 陈绍荣, 钱门宝, 李石柱. 基于受试者工作特征曲线评价询检法用于带绦虫病的诊断价值[J]. 中国血吸虫病防治杂志（中英文）, 2025, 37(4): 346-354.
[3]	魏春, 林祖锐, 杨中华, 周红宁, 周兴武, 杨锐. 中缅边境地区消除疟疾策略与疟疾联防联控合作探讨[J]. 中国血吸虫病防治杂志（中英文）, 2025, 37(1): 19-23,54.
[4]	周济华, 白少文, 施亮, 张键锋, 杜春红, 宋静, 张宗亚, 颜嘉琦, 吴安东, 董毅, 杨坤. 人工智能识螺系统识别湖北钉螺滇川亚种与拟钉螺的效能评价[J]. 中国血吸虫病防治杂志（中英文）, 2025, 37(1): 55-60,68.
[5]	白少文, 周济华, 董毅, 张键锋, 施亮, 杨坤. 基于EfficientNet⁃B4模型的云南省湖北钉螺滇川亚种视觉智能识别模型的建立[J]. 中国血吸虫病防治杂志（中英文）, 2024, 36(6): 555-561.
[6]	张宗亚, 杜春红, 张云, 王洪琼, 宋静, 周济华, 王丽芳, 孙佳昱, 沈美芬, 陈春琼, 江华, 颜嘉琦, 冯锡光, 王文雅, 钱沛君, 薛靖波, 李石柱, 董毅. 基于随机森林和最大熵模型的云南省钉螺潜在地理分布预测[J]. 中国血吸虫病防治杂志（中英文）, 2024, 36(6): 562-571,613.
[7]	周瑜, 杨淑娟, 杨媛, 和雁, 袁淑莲, 陈朝闻, 任天广. 2023年云南省龙陵县居民恙虫病防治知信行现状及影响因素[J]. 中国血吸虫病防治杂志（中英文）, 2024, 36(5): 507-513.
[8]	崔晓, 宋静 , 李春英, 王洪琼, 杜春红, 沈美芬, 杨早改, 史欣平, 李石柱, 董毅. 云南省血防专业人员钉螺与拟钉螺鉴别影响因素分析[J]. 中国血吸虫病防治杂志（中英文）, 2024, 36(5): 514-520.
[9]	张云, 王丽芳, 冯锡光, 吴明寿, 沈美芬, 江华, 宋静, 孙佳昱, 陈春琼, 颜嘉琦, 张宗亚, 周济华, 董毅, 杜春红. 云南省血吸虫病传播阻断历程与展望[J]. 中国血吸虫病防治杂志（中英文）, 2024, 36(4): 422-427.
[10]	陶香林, 马飞, 李政, 阚心蕊, 叶长江, 孙恩涛. 基于细胞色素b和内转录间隔区基因序列的4个棕脊足螨地理种群遗传变异分析[J]. 中国血吸虫病防治杂志（中英文）, 2023, 35(1): 22-.
[11]	丁春丽, 许建卫, 林祖锐, 许时燕, 崔鑫, 孙维江, 田光强, 李春华, 罗宗圣, 周耀武, 杨亚明. 云南省沧源县班老乡人群疟疾防治知识行为及其影响因素分析[J]. 中国血吸虫病防治杂志（中英文）, 2023, 35(1): 44-.
[12]	王丽明, 王卫群, 王红, 淳于纬训, 李娟, 向征, 李翠英. 立足云南省情开展《医学寄生虫学》课程思政的实践 [J]. 中国血吸虫病防治杂志（中英文）, 2022, 34(5): 537-.
[13]	宋静, 董毅, 杜春红, 张宗亚, 沈美芬, 张云, 周济华, 李石柱. 云南省钉螺螺壳形态学性状指标测量 [J]. 中国血吸虫病防治杂志（中英文）, 2022, 34(4): 341-.
[14]	陈凤, 李科荣, 李文豹, 田淑惠, 李萍, 赵银娇, 杨敬, 杨华, 罗秉荣, 马俊华, 郝明明, 陈绍荣, 刘榆华, 罗天鹏. 血吸虫病传播阻断地区环介导等温扩增技术检测钉螺血吸虫感染性效果[J]. 中国血吸虫病防治杂志（中英文）, 2022, 34(1): 81-.
[15]	吴方伟, 汪丽波, 李奔福, 严信留, 字金荣, 彭佳, 蔡璇, 保雪莹, 杨亚明. 2015年云南省人体土源性线虫感染调查 [J]. 中国血吸虫病防治杂志（中英文）, 2021, 33(5): 513-.