关键词: 疟疾, 消除, 参比实验室, 四川省

Abstract: Objective　To re⁃examine the diagnosis results of reported malaria cases in Sichuan Province from 2014 to 2020, so as to assess the malaria diagnostic capability of Sichuan Provincial Malaria Diagnostic Reference Laboratory. Methods　The blood and blood smear samples from reported malaria cases were collected by Sichuan Provincial Malaria Diagnostic Reference Laboratory from 2014 to 2020, and subjected to re⁃examinations using microscopy and nested PCR assay. The re⁃examination results were compared. Results　A total of 1 710 samples from reported malaria cases were re⁃examined by Sichuan Provincial Malaria Diagnostic Reference Laboratory from 2014 to 2020, and 1 634 samples were identified positive, with a positive coincidence rate of 95.56% （1 634/1 710） and a 92.29% (1 508/1 634) total coincidence rate of the Plasmodium species. The coincidence rates with P. falciparum, P. vivax, P. malariae and P. ovale were 99.48% (961/966), 97.07% (430/443), 83.05% (98/118) and 67.86% (19/28), respectively, and the coincidence rate was 91.81%（1 513/1 648） between microscopic and nested⁃PCR results. Conclusions　The capability of microscopists remains weak at grassroot medical institutions in Sichuan Province. Further training is required among microscopists to improve the malaria surveillance capability in Sichuan Province during the post⁃elimination stage.

Key words: Malaria, Elimination, Reference laboratory, Sichuan Province