关键词: 片形吸虫病； 大片吸虫； 肝片吸虫； 酶联免疫吸附试验； 敏感性； 特异性； 约登指数； 评价

Abstract:

Objective To evaluate the effect of 3 ELISA kits on detection of human fasciolasis. Methods Twenty?six serum samples from patients with fasciolasis，180 serum samples from patients with other parasitic diseases as well as 26 serum samples from healthy people were detected by ELISA kits which using soluble antigen of Fasciola gigantica，Fasciola hepatica（Fg?ELISA and Fh?ELISA）as well as IgG antigen ELISA detection kits made by DRG company in Germany. The effects of the 3 kits were eval? uated. Results The sensitivities of Fg?ELISA，Fh?ELISA，and DRG?ELISA were 100.0%，80.8%（95% CI：65.7%-95.9%） and 100.0%，respectively；the specificities of the three were 87.9%（95% CI：83.5%?92.4%），85.0% （95% CI：80.1%-89.9%） and 83.5%（95% CI：78.4%-88.6%），respectively，and Youden indexes of them were 0.88，0.66 and 0.84，respectively. The de? tection rate of Fg?ELISA（100%）was significantly higher than that of Fh?ELISA（80.8%） （P <0.05） . The A absolute value（A/ CO）of Fg?ELISA was 1.70，which was also significantly higher than the value of Fh?ELISA（1.18）（P < 0.000 1） . Conclusion Fg?ELISA has a good detection effect and low cost，and is more suitable than Fh?ELISA and DRG?ELISA for clinical sample tests as well as massive screening in fasciolasis endemic areas in southwest China.

Key words: Fasciolasis；Fasciola gigantica；Fasciola hepatica；Enzyme?linked immunosorbent assay（ELISA）；Sensitivity； Specificity；Youden index；Evaluation