关键词: 人芽囊原虫；白介素17；白介素23；肠黏膜；小鼠

Abstract:

Objective To study the expressions of interleukin?17（IL?17）and interleukin?23（IL?23）in the intestinal muco?
sa of BABL/C mice infected with Blastocystis hominis. Methods A total of 30 BABL/C mice were randomly divided into different
groups：an experimental group，an immunosuppressant group and a normal group. Each mouse of the experimental group and im?
munosuppressant group was administered intraperieneally with dexamethasone（2mg，gd，for 5 days）and one of the control group
was given physiological saline（0.2 ml）. In the experimental group，each mouse was infected with Blastocystis hominis（107 para?
sites per 0.5 ml）by the intragastric infusion method；in the immunosuppressant group and normal group，the mice were fed with
equal physiological saline. On the fifth day post?infection，the duodenum，jejunum，ileum and colon of the mice of the 3 groups
were taken out for the tissue section. The pathological changes of bowel mucosa were determined by HE staining，and the expres?
sions of IL?17 and IL?23 in different parts of bowel mucosa were determined by immunohistochemistry assay. Results The patho?
logical examinations showed intestinal mucosa had various degrees of inflammatory changes. The expressions of IL?17 and IL?23 in
the intestinal mucosa of the mice in the experimental group was significantly higher than those in the immunosuppressant group or
normal group（both P < 0.05）. The expressions of IL?17 and IL?23 in the intestinal mucosa of the mice in the immunosuppressant
group were similar to those in the normal group（P > 0.05）. The expression of IL?17 in the duodenum or jejunum or colon of the
mice was significantly higher than that in the ileum in the experimental group（ P <0.05）. The expression of IL?23 in the duode?
num or jejunum of the mice was significantly higher that that in the ileum or colon in the experimental group（P < 0.05）. Conclu?
sions IL?17 and IL?23 are highly expressed in the intestinal mucosa of the mice infected with Blastocystis hominis. IL?23 may al?
so be involved in the immunomodulatory effects of Blastocystis hominis infection，which plays a mutual regulatory role with IL?17.

Key words: Blastocystis hominis；Interleukin?17（IL?17）；Interleukin?23（IL?23）；Intestinal mucosa；Mouse