关键词: 日本血吸虫病；CD4+CD25+Treg细胞；细胞免疫；鄱阳湖区

Abstract:

Objective To observe the characterization of cellular immune of schistosomiasis japonica patients in low endemic
area of Poyang Lake region. Methods The schistosomiasis patients who were positive by stool detections were selected as study
subjects，and the individuals who were negative by stool detections in three consecutive years were selected as negative controls.
The CD4+T cell，CD8+T cell and CD4+CD25+Treg in venous blood of the study subjects were detected by using flow cytometry. In
vitro，cytokine（IFN?γ and IL?10）production of PBMC in response to soluble egg antigen（SEA）and soluble worm antigen prepa?
ration（SWA）were measured by ELISA. Results The levels of CD4+CD25+Treg in individuals who were positive by stool detec?
tions（5.99±1.60）% were higher than those of the negative（5.04±1.64）%，and the difference was significant（t = 3.261，P ＜
0.01）. One year after the treatment，the levels of IFN?γ were significantly higher（Stimulated by SEA before and after the treat?
ment，the mean levels were 121.97 and 2 564.03 pg/ml，respectively；stimulated by SWA before and after treatment，the mean
levels were 102.89 and 646.26 pg/ml，respectively）and IL?10 were significant lower in patients who were positive by stool detec?
tions（Stimulated by SEA before and after treatment，the mean level were 294.75 and 122.43 pg / ml，respectively；stimulated by
SWAP before and after treatment，the mean cytokine levels were 110.10 and 31.85 pg/ml，respectively）. Conclusion The in?
crease of induced CD4+CD25+Treg is one of causes that down?regulate cellular immune of human body after infection with Schistoso?
ma japonicum.

Key words: Schistosomiasis japonica；CD4+CD25+Treg；Cellular immune；Poyang Lake region