关键词: 亚洲绦虫； 带绦虫； COX1；同源性； 遗传距离；广西壮族自治区

Abstract:

Objective To analyze the COX1 sequences of Taenia isolates from four areas of Guangxi Zhuang Autonomous Re? gion，and to understand the distribution of Taenia asiatica in Guangxi. Methods Patients with taeniasis in Luzhai，Rongshui，Ti? andong and Sanjiang in Guangxi were treated by deworming，and the Taenia isolates were collected. Cyclooxygenase?1（COX1）se? quences of these isolates were amplified by PCR，and the PCR products were sequenced by T?A clone sequencing. The homogene? ities and genetic distances were calculated and analyzed，and the phylogenic trees were constructed by some softwares. Meanwhile， the COX1 sequences of the isolates from the 4 areas were compared separately with the sequences of Taenia species in GenBank. Results The COX1 sequence of the 5 Taenia isolates collected had the same length of 444 bp. There were 5 variable positions be? tween the Luzhai isolate and Taenia asiatica，the homogeneity was 98.87% and their genetic distance was 0.011. The phylogenetic tree analysis revealed that the Luzhai isolate and Taenia asiatica locating at the same node had a close relationship. The homogenei? ty between Rongshui isolate A and Taenia solium was 100%，while the homogeneity of Rongshui isolate B with Taeniasis saginata and Taenia asiatica were 98.20% and 96.17%，respectively. The homogeneities of the Tiandong and Sanjiang isolates with Taenia solium were 99.55% and 96.40%，respectively，and the genetic distances were 0.005 and 0.037，respectively. The homogeneity between the Luzhai isolate and Taeniasis saginate was 96.40%. Conclusion Taenia asiatica exists in Luzhai and Taenia solium and Taenia saginata coexist in Rongshui， Guangxi Zhuang Autonomous Region.

Key words: Taenia asiatica； Taenia；COX1； Homogeneity；Genetic distance；Guangxi Zhuang Autonomous Region