Abstract:

Objective To observe the change of liver sinusoid in BAI.B/c mice infected with Schistosoma japonicum and explore its relationship between the degree of hepatic fibrosis and liver function. Methods A model was established in BALB/c mice infected with cercariae of Schistosoma japonicum. The liver specimens of mice were used for pathological examination with routine and pi cric acid-sirius red staining to know the degree of hepatic fibrosis by semi-quantity. The expressions of C-IV and vWF in liver sinusoid were assayed through the immuhistochemistry staining. The serum ALT and AST were detected by the automatic biochemistry analyzer, and the ultrami crostructure of the liver tissue was observed by a transmission electron microscope. Results The number and diameter of fenestrations in SEC reduced 4 weeks after the infection. The fcnestrations were disappeared and the base membrane established 8 weeks after the infection. The expressions of C-IV and vWF in liver sinusoid and the degree of hepatic fibrosis increased with the time after infec tion. The levels of serum ALT and AST were not correlated with the infection time. Conclusions The phenotype alteration of the hepatic sinusoidal endothelium may be a vital issue triggering the liver fibrosis induced by Schistosoina japoncium.

Key words: Schistosomiasis, Liver sinusoid, Liver fibrosis, Mouse

摘要：

目的 观察感染日本血吸虫BALB/c小鼠肝窦动态变化，及其与肝纤维化和肝细胞损伤的关系。方法 建立感染日本血吸虫BALB/c小鼠肝纤维化动物模型，肝标本行常规病理染色和天狼猩红染色，进行肝纤维化程度的半定量分析，免疫组化染色检测肝窦IV型胶原(C-IV)和第Ⅷ因子相关抗原(vWF)的表达，测血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)，另每组取2只小鼠新鲜肝组织进行透射电镜观察。结果 感染小鼠肝窦内皮细胞(SEC)第4周窗孔减少、变小，第8周窗孔消失及SEC下基底膜(BM)形成，肝窦C-IV和vWF随感染时间的延续表达逐渐增强，肝纤维化程度也逐渐增高，血清ALT和AST与感染时问无关。结论 血吸虫感染所引起的小鼠SEC表型改变可能是其诱导肝纤维化的始动机制之一。

关键词: 日本血吸虫病；肝窦；肝纤维化；小鼠