Abstract:

Objective To get a molluscicide from Reineckea carnea which is effective on Oncomelania hupensis but has low poison on fish. Methods Methyl soaking, column chromatography and HPLC were used for isolation of molluscicidal component from Reineckea carnea. Results The snail mortality with Reineckea carnea powder was 86. 0% for 72 h immersion and 100. 0% for 120 h at the concentration of 17. 5 mg/L, but the fish mortality was 0 at the concentration of 130 mg/L, indicating that Reineckea carnea had different effects on the two kinds of animals . The fraction A was obtained from Reineckea carnea by methyl immersion, column chromatography. The snail mortality with A was 84. 4% for 144 h at the concentration of 10 mg/L, indicating that A had high molluscicidal effect on Oncomelania hupensis. After A being isolated by HPLC, 99. 98% RC- I was obtained from it . RC- I was determined by API2000 LC/MS and other spectrum, and it was deduced to be one of steroidal glycosides. Conclusion Reineckea carnea contains a high effective molluscicidal component.

Key words: Reineckea carnea, Oncomelania hupensis, Molluscicide, Isolate

摘要：

目的 从吉祥草中提取分离杀灭钉螺活性高和对鱼毒性低的化合物。方法 使用吉祥草 200孔/25.4 mm原粉，经甲醇浸提，正丁醇萃取，再经硅胶柱层析和HPLC纯化，分离得到目标化合物。结果 吉祥草原粉浸杀钉螺：浓度在17.5 mg/L，经72 h和120 h其死亡率分别达到86. 0%和 100．0%；浓度在130 mg/l。，经168 h鱼死亡率为0。原粉经提取分离得到A组分浓度在10 mg/L浸杀钉螺，经144 h其死亡率为84, 4%，显示A组分对钉螺有较强的毒杀效应。A组分经HPLC分离纯化得到99. 98%纯度的RC-I，API 2000质谱仪LC/MS联机测定其分子量，结合其他光谱分析推定RC-I属甾体化合物。结论 吉祥草中有杀螺作用较强的化合物。

关键词: 吉祥草；钉螺；杀螺化合物；提取分离