Abstract: Objective Objective To observe the preservation time and activity of miracidium hatching from schistosome eggs preserved in different solutions，so as to obtain the optimal preservation conditions and then provide quality control products for field appli? cation. Methods Methods The rectum stool of rabbits infected with Schistosoma japonicum was collected and the coarse fecal residue was removed with a series of sample sieves of 80，100，160 and 200 meshes respectively，and then the schistosome eggs were concentrated with the sample sieve of 260 meshes. The concentrated eggs were preserved in 0.9% sodium chloride solution， 1.2% sodium chloride solution，phosphate buffered saline solution（PBS，PH 7.2），1.0% sucrose solution，and Mili?Q water， respectively，and then were conserved in a 4 ℃ refrigerator and 15 ℃ constant temperature incubator，respectively. The pre? served eggs were hatched in different time（7?day interval），the vitality and quantity of the miracidia were observed，and the hatching rates were calculated. Results Results Under the condition of 4 ℃，the hatching rates of eggs dropped to 0 in 1.0% sucrose so? lution and 1.2% sodium chloride solution at the 49th and 126th day，respectively，and the hatching rates of eggs in the 0.9% so? dium chloride solution and PBS solution dropped to 10% at the 112th day，and the activity of miracidium was weakened since 140th. In the Mili?Q water，the hatching rate dropped less than 10% at the 196th day and the activity of miracidia was weakened since the 280th day. Under the condition of 15 ℃，the hatching rate of eggs in different solutions gradually dropped to 0 from the 49th day to 105th day. Conclusion Conclusion The eggs preserved in Mili?Q water at the temperature of 4 ℃ can be used as the positive reference for hatching tests within 196 days.

Key words: Schistosoma japonicum；Egg；Hatching test；Reference；Preservation

摘要： 目的 目的 观察血吸虫活虫卵在不同保存液中的保存时间及孵化毛蚴的活力， 获得血吸虫活虫卵最佳保存条件， 探 索用作孵化参考品的可行性。方法 方法 收集人工感染血吸虫家兔直肠粪便， 经80、 100、 160孔/25.4 mm和200孔/25.4 mm 分样筛过滤去除粗粪渣， 260孔/25.4 mm收集血吸虫活虫卵， 于0.9% NaCl溶液、 1.2% NaCl溶液、 磷酸盐缓冲液 （PBS， pH 7.2）、 1.0%蔗糖溶液和Mili?Q水5种保存液中混匀， 分别于4 ℃和15 ℃恒温保存。每隔7 d对保存虫卵进行孵化， 观察毛 蚴活力并计数。结果 结果 在4 ℃条件下， 1.0%蔗糖溶液和1.2% NaCl溶液中虫卵孵化率分别于49 d后和126 d后降至0； 0.9% NaCl溶液和PBS保存液中虫卵孵化率于112 d后降至10%以下， 140 d后孵出的毛蚴活力较差； Mili?Q水保存的虫卵 孵化率于196 d后降至10%以下， 280 d后孵出毛蚴， 活力明显下降且不利于观察。在15 ℃条件下， 各保存液中虫卵的孵 化率分别在49 ~ 105 d之间逐渐降至0。结论 结论 实验室以Mili?Q水为血吸虫活虫卵保存液， 置4 ℃恒温保存， 196 d之内 的冷藏虫卵具有用作孵化阳性参考品的价值。

关键词: 日本血吸虫； 虫卵； 孵化试验； 参考品； 保存