Abstract:

in 5 μl sera. Methods A soluble egg antigen（SEA）from Schistosoma japonica was separated with sephacryl S?
300 column to retain the active antigen fractions to the antibody of schistosome. The optional reaction system and detection kit with
5 μl sera were established by improving conjugated concentration and formulas of sample buffer and labeled solution. Results
The sensitivity of detecting schistosomiasis patients whose stool examinations for schistosome eggs were positive was 93.7%，the
specificity to health population 97.1%，the cross reaction rate to patients with paragonimiasis 5.6%. The Youden’s index value
was 0.91. There was 96.1% crude coincidence of GICA and ELISA in detecting 507 cases of floating population and the Kappa val?
ue was 0.81. Conclusion GICA kit is practical for detection of schistosomiasis in the field because of its advantages such as
smaller serum needed，the high sensitivity，lower cross reaction rate and spread application for human and animals.

Key words: Schistosomiasis；Soluble egg antigen（SEA）；Smaller serum；Antibody；Gold immunochromatography assay（GICA）；

摘要：

目的 研制血清用量少的血吸虫抗体检测层析卡。方法 采用Sephacryl S?300HR柱层析分离血吸虫可溶性虫卵抗原（SEA），保留具有特异性反应活性的抗原蛋白组分。并研究标记浓度及稀释剂配方、样品缓冲液成分，组成最佳反应体系，建立血清用量5 μl的血吸虫抗体检测层析卡。结果 血吸虫抗体检测层析卡对血吸虫虫卵阳性病人的敏感性为93.7%，EPG <24的低感染度检出率达91.3%，健康人群的特异性为97.1%，并殖吸虫病人的交叉反应率为5.6 %，Youden指数为0.91。与ELISA平行检测流动人口的总符合率为96.1％，Kappa值为0.81。结论 血吸虫抗体检测层析卡具有血清用量少、敏感性高、特异性强、 交叉反应低、应用范围广等特点，具有现场实用价值。

关键词: 血吸虫病；可溶性虫卵抗原；微量血清；抗体；层析卡