Abstract:

Objective To understand the characterization and levels of antibody isotype responses to soluble egg antigen（SEA）and adult worm antigen（AWA）of Schistosoma japonicum in schistosomiasis patients in Poyang Lake region. Methods Antibody isotype IgG，IgG1，IgG2，IgG3，IgG4，IgE，IgM and IgA responses to SEA and AWA in the sera of individuals from endemic and non.endemic areas were determined by indirect ELISA. Results The levels of antigen.specific antibody isotypes in the sera of patients with egg positive were significantly higher than those of people with egg negative and normal control population from non.endemic area（P < 0.001），and except for anti.SEA IgG2 and anti.AWA IgG4 and IgE antibodies，the levels of antibody isotypes in the sera of people with egg negative in endemic area were significantly higher than those of normal control population from non.endemic area（P < 0.05）. In the population with egg positive，children had higher levels of anti.SEA IgM and IgG1 and anti.AWA IgM antibody，and lower AWA specific IgG than adults. There was no significant difference between the male and female except for the male had higher levels of IgG（F = 4.677，P = 0.032）and IgG1（F = 5.55，P = 0.020）against AWA. Three0.029，P = 0.044），and almost all the antibody levels decreased significantly（P < 0.05）twelve months post.treatment，except for SEA specific IgE and IgM and AWA specific IgG4 and IgM antibodies. Conclusions The levels of antigen.specific antibody isotypes in the sera of patients with egg positive are significantly higher than those of people with egg negative and normal control population from non.endemic area. Almost all of antibody levels decrease significantly twelve months post.treatment. The levels of antibody isotypes in the sera of people with egg negative in endemic area are significantly higher than those of population from non.endemic area，giving a clue that the selection of negative control of schistosomiasis diagnostic kit should be noticed.

Key words: Schistosoma japonicum；Antibody isotype；Soluble egg antigen；Adult worm antigen

摘要：

目的了解鄱阳湖区血吸虫病人特异性同种型抗体反应水平及特征。方法以可溶性虫卵抗原（SEA）和成虫抗原（AWA）为检测抗原，采用间接ELISA法检测血吸虫病流行区及非流行区人群血清中8种特异性同种型抗体（IgG、IgG1、IgG2、IgG3、IgG4、IgE、IgM、IgA）水平。结果血吸虫病流行区粪检阳性人群特异性同种型抗体水平显著高于流行区粪检阴性人群和非流行区人群（P均＜0.001），流行区粪检阴性人群除SEA和AWA特异性IgG2、IgG4和抗AWA.IgE抗体外，其他同种型抗体水平均显著高于非流行区人群（P均＜0.05）。流行区粪检阳性人群中，儿童SEA、AWA特异性IgM和SEA.IgG1水平显著高于成人，AWA特异性IgG水平则显著低于成人（F = 6.136，P = 0.014）；男性除AWA特异性IgG（F = 4.677，P = 0.032）和IgG1（F = 5.55，P = 0.020）显著高于女性外，其他抗体同种型之间差异均无统计学意义（P均＞0.05）。治疗3个月后，SEA抗原特异性IgG1、IgG3、IgG4抗体水平显著下降（P ＜ 0.001，P = 0.029，P = 0.044）；治疗12个月后，除SEA特异性IgE、IgM和AWA.IgG4、IgM外，其他抗体均显著低于治疗前水平（P均＜0.05）。结论血吸虫病流行区粪检阳性人群血吸虫特异性同种型抗体水平显著高于流行区粪检阴性人群和非流行区人群，治疗12个月后，大部分同种型抗体水平显著下降；流行区粪检阴性人群血吸虫特异性抗体水平高于非流行区人群。抗体诊断试剂盒研制应注意阴性参考血清的选择。

关键词: 日本血吸虫；同种型抗体；可溶性虫卵抗原；成虫抗原