Abstract: Objective　To investigate the species of ticks in Suizhou City, Hubei Province, so as to provide insights into management of ticks and tick⁃borne diseases. Methods　During the period between May 2023 and June 2024, livestock breeding farms and vegetation neighboring the place of residence of confirmed and suspected patients with tick⁃borne disease were selected as sampling points in rural areas from Yindian Township, Gaocheng Township, Wanhe Township, Wushan Township, Xiaolin Township, Xihe Township, Hedian Township and Beijiao Street in Suizhou City, Hubei Province, where confirmed and suspected cases with tick⁃borne diseases had been reported. The parasitic ticks on the body surface of free⁃range livestock were captured with tweezers in livestock breeding farms, and free ticks on the vegetation surface were captured with the flagging method. Morphological identification of tick samples was performed under a microscope, and the gender and developmental stage of ticks were determined. One engorged adult tick, 2 to 3 blood⁃feeding but non⁃engorged adult ticks, 10 to 15 unfed female ticks, 15 to 20 unfed male ticks, and 30 to 40 tick nymphs or larvae were assigned into a group, respectively. Genomic DNA was extracted from tick samples in each group, and mitochondrial 16S rRNA gene was amplified. Sequence analysis was performed with the DNASTAR software, and phylogenetic analysis was performed using the software MEGA 7.0. In addition, the phylogenetic tree was generated using the maximum likelihood method based on the Kimura 2 parameter model. Results　A total of 2 438 ticks were captured from Suizhou City, Hubei Province during the period between May 2023 and June 2024, including 595 free ticks and 1 483 parasitic ticks. Three developmental stages of ticks were captured, including larvae, nymphs, and adults, and 75.18% (1 899/2 438) of captured ticks were adult, in which 79.04% (1 501/1 899) were female. Morphological and molecular biological assays identified one family, three genera and four species of captured ticks, including 2 425 Haemaphysalis longicornis ticks (99.47%) and one H. flava tick (0.04%) of the genus Haemaphysalis, 11 Rhipicephalus microplus ticks (0.45%) of the genus Rhipicephalus, and one Ixodes sinensis tick (0.04%) of the genus Ixodes in the family Ixodidae. Phylogenetic analysis revealed that the H. longicornis sequence (SZ49) in this study was clustered with sequences from Yunnan Province (GenBank accession number: MH024510.1), Hebei Province (GenBank accession number: MK450606.1) and Henan Province (GenBank accession number: MZ230645.1) into a clade, and the H. flava sequence (SZ19) in this study was clustered with sequences from Japan (GenBank accession number: MW064044.1), South Korea (GenBank accession number: ON629585.1), and Jiangsu Province (GenBank accession number: PP494741.1) and Hebei Province of China (GenBank accession number: MH520685.1) into a clade, while the R. microplus sequence (SZ8) in this study was clustered with the sequences from India (GenBank accession number: MK621328.1), and Henan Province (GenBank accession number: MT555307.1) and Guizhou Province of China (GenBank accession number: PP446801.1) into a clade. The sequence of I. sinensis (SZ23) in this study had 99.51% homology with that (GenBank accession number: OM368265.1) of ticks sampled from Wuhan City, Hubei Province. Conclusion　There are four tick species of H. longicornis, H. flava, R. microplus and I. sinensis in Suizhou City, Hubei Province, and H. longicornis is the dominant species. H. flava is firstly recorded in Suizhou City. 

Key words: Tick, Haemaphysalis longicornis, Haemaphysalis flava, Morphological identification, Phylogenetic analysis, Suizhou City

摘要： 目的　了解湖北省随州市蜱的种类，为当地蜱及蜱传疾病防控提供科学依据。方法　2023年5月—2024年6月，在湖北省随州市既往报告确诊及疑似蜱传疾病病例的殷店镇、高城镇、万和镇、吴山镇、小林镇、淅河镇、何店镇及北郊街道管辖的农村地区，以病例居住地为中心，选取附近家畜养殖场及植被作为采样点。用镊子夹取养殖场散养家畜体表寄生蜱，用布旗法采集植被表面的游离蜱。于显微镜下对蜱样本进行形态学鉴定，并确定其性别及生长阶段。分别将1只饱血成蜱，2 ~ 3只吸血但未饱血成蜱，10 ~ 15只未吸血雌蜱，15 ~ 20只未吸血雄蜱，30 ~ 40只若蜱、幼蜱分为1组，提取各组蜱样本DNA，扩增蜱线粒体16S rRNA基因，采用DNASTAR软件分析获得的序列；采用MEGA 7.0软件进行系统发育分析，采用最大似然法（maximum likelihood method）并基于Kimura 2参数模型构建系统发育树。结果　2023年5月—2024年6月，累计于湖北省随州市采集蜱2 438只，其中游离蜱595只，寄生蜱1 843只；所采集的蜱样本包括幼蜱、若蜱、成蜱3个生长阶段，其中成蜱占75.18%（1 899/2 438）；雌性成蜱占成蜱总数的79.04%（1 501/1 899）。经形态学及分子生物学鉴定，采集的蜱隶属于1科3属4种，包括硬蜱科血蜱属长角血蜱2 425只（占99.47%）和褐黄血蜱1只（占0.04%）、扇头蜱属微小扇头蜱11只（占0.45%）、硬蜱属中华硬蜱1只（占0.04%）。经系统发育分析，本研究采集的长角血蜱序列（SZ49）与来自云南省（MH024510.1）、河北省（MK450606.1）、河南省（MZ230645.1）的序列汇集于一簇，褐黄血蜱序列（SZ19）与来自日本（MW064044.1）、韩国（ON629585.1）及我国江苏省（PP494741.1）、河北省（MH520685.1）的序列汇集于一簇，微小扇头蜱序列（SZ8）与来自印度（MK621328.1）及我国河南省（MT555307.1）、贵州省（PP446801.1）的序列汇集于一簇，中华硬蜱序列（SZ23）与来自湖北省武汉市（OM368265.1）的序列同源性达99.51%。结论　湖北省随州市存在长角血蜱、褐黄血蜱、微小扇头蜱和中华硬蜱4种蜱，其中长角血蜱为优势蜱种，褐黄血蜱为随州市首次发现。

关键词: 蜱, 长角血蜱, 褐黄血蜱, 形态学鉴别, 系统发育分析, 随州市