Abstract: Toxoplasma gondii is an obligatory intracellular parasite which infects a variety of warm⁃blooded animals and causes toxoplasmosis. Toxoplasmosis seriously endangers human health and animal husbandry production. As one of the effective gene editing tools, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR⁃associated proteins (Cas) system has been widely used for knockout of genes in T. gondii. This review summarizes the applications of the CRISPR/Cas9 technology in vaccines against single⁃ and double⁃gene deletion strains of T. gondii, so as to provide insights into development of toxoplasmosis vaccines.

Key words: Toxoplasma gondii, CRISPR/Cas9 technology, Vaccine, Gene?deletion strain

摘要： 刚地弓形虫是专性细胞内寄生虫，可感染多种温血动物，引起弓形虫病。弓形虫病严重危害人体健康及畜牧业生产。成簇规律间隔短回文重复序列（clustered regularly interspaced short palindromic repeats，CRISPR）及其相关蛋白（CRISPR⁃associated protein，Cas）系统（CRISPR/Cas系统）是有效的基因编辑工具之一，广泛应用于弓形虫基因敲除。本文综述了CRISPR/Cas9技术在弓形虫病单基因和双基因缺失株疫苗研究中的应用，旨在为弓形虫病疫苗研制提供参考。

关键词: 弓形虫, CRISPR/Cas9技术, 疫苗, 基因缺失株