Abstract: Objective　To analyze the changes of small molecular metabolites in the larvae of a deltamethrin⁃sensitive strain of Anopheles sinensis following exposure to deltamethrin, so as to provide the scientific basis for investigating the metabolic pathway and screening metabolic markers of deltamethrin in An. sinensis. Methods　The 50% and 75% lethal concentrations (LC50 and LC75) of deltamethrin against the larvae of a deltamethrin⁃sensitive strain of An. sinensis were calculated in laboratory. The type and content of An. sinensis larvae metabolites were detected using high performance liquid chromatography and mass spectrometry (LC⁃MS/MS) following exposure to deltamethrin at LC50 and LC75 for 30 min and 24 h, and the changes of metabolites were analyzed. Results　The LC50 and LC75 values of deltamethrin were 4.36 × 10-3 μg/mL and 1.12 × 10-2 μg/mL against the larvae of a deltamethrin⁃sensitive strain of An. sinensis. Following exposure of the larvae of a deltamethrin⁃sensitive strain of An. sinensis to deltamethrin at LC50 and LC75 for 30 min, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, fatty acyl and pyrimidine nucleotides, with reduced glucose levels. Following exposure for 24 h, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, aliphatic acyl and purine nucleotides, with increased glucose level detected. Conclusion　Carbohydrate, carboxylic acid and its derivatives, fatty acyls, amino acids and their derivatives may play important roles in deltamethrin metabolism in the larvae of a deltamethrin⁃sensitive strain of An. sinensis.

Key words: Anopheles sinensis, Larvae, Deltamethrin, Metabolism

摘要： 目的　分析实验室溴氰菊酯敏感品系中华按蚊幼虫暴露溴氰菊酯后机体内小分子代谢物变化，为研究中华按蚊溴氰菊酯杀虫剂代谢通路、筛选代谢标志物提供科学依据。方法　测定实验室溴氰菊酯敏感品系中华按蚊幼虫溴氰菊酯半数致死浓度（LC50）和75%致死浓度（LC75）浓度，分别暴露于LC50、LC75浓度下30 min和24 h，运用液相色谱串联质谱（LC⁃MS/MS）技术检测幼虫代谢物种类和含量，分析代谢物变化趋势。结果　溴氰菊酯对中华按蚊溴氰菊酯敏感品系幼虫的LC50和LC75值分别为4.36 × 10⁃3 μg/mL和1.12 ×10⁃2 μg/mL。中华按蚊溴氰菊酯敏感品系幼虫暴露于溴氰菊酯30 min后，LC50、LC75两浓度组差异代谢物主要集中于有机氧化合物、羧酸及其衍生物、脂肪酰基、嘧啶核苷酸等，其中葡萄糖水平在溴氰菊酯接触组中浓度降低；暴露24 h后，差异代谢物主要集中于有机氧化合物、羧酸及其衍生物、脂肪族酰基、嘌呤核苷酸等，其中葡萄糖水平在溴氰菊酯接触组中浓度升高。结论　中华按蚊溴氰菊酯敏感品系幼虫暴露于溴氰菊酯后，代谢产物变化提示糖类和羧酸及其衍生物、脂肪族酰基类、氨基酸及其衍生物可能在溴氰菊酯代谢过程中发挥重要作用。　

关键词: 中华按蚊, 幼虫, 溴氰菊酯, 代谢