Abstract: Objective Objective To diagnose the first imported case of Plasmodium ovale infection by laboratory detection. Meth Meth? ? ods ods The epidemiological data and blood samples of the case were collected，and the samples were detected by the microscopic examination，rapid diagnostic test（RDT）and nested PCR. Results Results The patient was a construction worker backing from Con? go，Africa. He experienced the symptoms of irregular fever and weakness one month after returning in Lingyang Town，Junxian County. The results of RDT only suggested no?Plasmodium falciparum infection. Under the microscope，it was seen that the in? fected RBC were obviously disfigured and in irregular shape，the ring forms were thick and big，and also thick granulas in big trophozoite stage and schizont stage were found. The results of PCR showed that the size of amplified product was about 800 bp， which was conformed to that of P. ovale. Conclusion Conclusion Though microscopic examination is the golden standard for malaria diag? nosis，as P. ovale is difficult to be identified under microscope，the microscopic method combined with PCR test can be used for definite diagnosis.

Key words: Plasmodium ovale；Imported case；Microscopy；Rapid diagnostic test；Nested PCR

摘要： 目的 目的 对日照市首例输入性卵形疟病例进行实验室检测， 以明确诊断。方法 方法 收集患者流行病学资料和血样， 分别进行疟疾快速诊断检测 （RDT）、 疟原虫镜检及巢式PCR检测。结果 结果 患者从非洲刚果金务工返乡， 回居住地莒县陵 阳镇1个月后出现不规则发热、 乏力。RDT检测提示为非恶性疟原虫感染。血涂片镜下可见感染红细胞明显变形， 呈多 种不规则形状； 环状体粗大， 可见大滋养体期和裂殖体期疟原虫。巢式PCR扩增基因产物长度约800 bp， 与卵形疟原虫 相符。综合上述结果， 该病例诊断为单一卵形疟原虫感染。结论 结论 虽然镜检是疟疾诊断的金标准， 但卵形疟原虫在镜下 很难鉴别， 结合 PCR检测结果可明确诊断。

关键词: 卵形疟； 输入性病例； 镜检； 快速诊断检测； 巢式PCR