Abstract: Objective Objective To explore the correlation between the genetic dissimilarity and heterozygosity of mates and the patho? genicity of Schistosoma japonicum in the definitive host. Methods Methods By using seven microsatellite loci markers，S. japonicum genotyping of sixteen pairs randomly mated was performed，the genetic dissimilarity and heterozygosity were calculated between the mates，and the correlation between the genetic dissimilarity and heterozygosity of the mates and the pathogenicity of S. japon? icum in the definitive host was evaluated. Results Results There was a significant correlation between the genetic similarity of S. ja? ponicum mates and the mean number of eggs per worm pair in the liver and intestinal tissue（r = 0.501 6，P < 0.05；r = 0.796 5，P < 0.01，respectively）and the hatching rate of deposited eggs in the liver（r = 0.508 3，P < 0.05），respectively. There was no correlation between the genetic similarity of the mates and hepatosplenomegaly per worm pair（r = 0.109 5，P > 0.05；r = 0.265 3，P > 0.05，respectively）and the average diameter of granuloma in the liver（r = -0.272 7，P > 0.05），respec? tively. There was no correlation between the heterozygosity of the mates and all the pathological parameters of S. japonicum in the definitive host（P > 0.05） . Conclusions Conclusions There is the correlation between the genetic dissimilarity of the mates and the pathogenicity of S. japonicum in the definitive host，and the genetic dissimilarity is greater，pathogenicity is weaker. There is no correlation between heterozygosity of the mates and the pathogenicity of S. japonicum in the definitive host.

Key words: Schistosoma japonicum, Genetic dissimilarity, Heterozygosity, Pathogenicity, Correlation

摘要： 目的 目的 探讨日本血吸虫配对个体间遗传学差异与其对终宿主致病性的相关性。方法 方法 利用7个微卫星位点标 记对16组随机配对的日本血吸虫雌雄成虫进行基因分型， 计算成虫间的遗传相似性和杂合度差值， 评价其与血吸虫对 终宿主致病性的关系。结果 结果 日本血吸虫配对个体间遗传相似性与平均每对成虫肝脏沉积虫卵数 （r = 0.501 6， P < 0.05）、 平均每对成虫肠组织沉积虫卵数 （r = 0.796 5， P< 0.01） 及肝脏沉积虫卵孵化率显著相关 （r = 0.508 3， P < 0.05）， 与 平均每对成虫所引起的肝脾肿大 （r肝肿大= 0.109 5， P肝肿大 > 0.05； r脾肿大= 0.265 3， P脾肿大 > 0.05） 及肝脏肉芽肿平均直径 （r = -0.272 7， P > 0.05） 均无相关性； 配对个体间的杂合度与终宿主各病理指标间均无相关性 （P均> 0.05）。结论 结论 日本 血吸虫配对个体间的遗传差异性与其对终宿主的致病性相关， 并且遗传差异越大、 致病性越弱； 配对个体间的杂合度与 其对终宿主的致病性无关。

关键词: 日本血吸虫, 基因差异性, 杂合度, 致病性, 相关性