Abstract: Objective Objective To separate and purify intrahepatic macrophages from Microtus fortis（Mf）and identify its phagocy? tosis. Methods Methods The intrahepatic macrophages from Mf were separated and purified by perfusion，collagenase digestion and density gradient centrifugation. The function of the cells was identified by FACS analysis and ink phagocytosis activity. Results Results The macrophage cells from the liver of Mf were obtained. These cells were bright and circular，and grew adhering to the wall. The proportion of the living cells was 95%. The binding rate of these cells from Mf with anti?mouse CD14 antibody（Clone，Sa2? 8）was about 50% of the rate of macrophage from C57BL/6 mice with this monoclonal antibody. The result of ink?phagocytosis ex? periment of macrophage cells from the liver of Mf was positive. Conclusion Conclusion The method above mentioned is useful to separate and purify macrophage from the liver of Mf. The study builds the foundation for further research on macrophages of Mf against Schistosoma japonicum.

Key words: Microtus fortis； Schistosoma japonicum； Macrophage； Isolation； Identification； Innate immune

摘要： 目的 目的 对东方田鼠 （Microtus fortis，Mf） 肝内巨噬细胞进行分离纯化和功能鉴定。方法 方法 采用在体灌注、 胶原 酶消化及梯度密度离心相结合的方法分离Mf肝内巨噬细胞， 并采用流式和墨汁吞噬功能实验进行巨噬细胞的功能鉴 定。结果 结果 通过此方法获得Mf肝脏来源的巨噬细胞， 分离的细胞呈透亮圆形， 贴壁生长， 活细胞比例为95%。Mf来源的 巨噬细胞与抗鼠CD14流式单抗 （Clone：Sa2?8） 阳性结合率是小鼠来源巨噬细胞与该抗体结合率的50%左右。Mf肝脏来 源的巨噬细胞墨汁吞噬实验阳性。结论 结论 该方法能有效地分离纯化Mf肝内巨噬细胞， 可为进一步研究东方田鼠肝脏巨 噬细胞抗血吸虫机制奠定基础。

关键词: 东方田鼠； 日本血吸虫； 巨噬细胞； 分离； 鉴定； 天然免疫