关键词: 血吸虫病, 间接血凝试验试剂盒, 诊断效能

Abstract:

Objective To evaluate the clinical diagnostic efficiency of Indirect Hemagglutination Assay Kit (IHA kit) for antibody detection of schistosomiasis japonica. Methods Schistosomiasis examinations by parasitological method were conducted in the schistosomiasis endemic areas in Jiangxi, Anhui and Hubei provinces, respectively. A total of 371 serum samples of schistosomiasis patients and 761serum samples of non-patients without history of schistosome infection and infested water contact were collected. All the sera were detected by using IHA Kit. The diagnostic efficiency indicators of the kit, including sensitivity, specificity, positive predicted value, negative predicted value etc. were calculated. Results The sensitivity and specificity of the kit were 95.1% (95% confidence interval was between 92.9% and 97. 3%) and 97.8% (95% confidence interval was between 96.8% and 98.8%), respectively; the positive and negative predicted values were 95.4% and 97.6%, respectively. No statistically significant difference was found in the prevalence of IHA positive detecting by the kit among the groups with different EPG levels (≤40, 41－100, ＞100). Also, no significant difference was observed in sensitivity and specificity among the different age groups. Conclusions The IHA kit shows a relative high efficiency for diagnosis and can be used for mass screening of schistosomiasis in field sites.

Key words: Schistosomiasis, Indirect Hemagglutination Assay kit (IHA kit), Diagnostic efficiency