关键词: 血吸虫病；诊断；粪孵法；质量控制；现场评估

Abstract: Objective To evaluate the pathogenic detection capacity of county?level organizations in Jiangsu Province, and to improve the field schistosomiasis detection capacity of professional personnel, thus to provide the technical support for the construction of quality control system of schistosomiasis field detection. Method The eggs of Schistosoma japonicum were obtained from rabbit schistosomiasis models and were produced into suspensions at four different concentrations. The county?level workers were invited to hatch the eggs, and the accuracy, detection error rate and omission rate were compared between the detection results and the standard results. The single?blind method was used in the capacity examination. Result A total of 560 suspensions were detected by 28 counties (districts, cities), and 283 positive samples and 203 negative samples were detected. The total accuracy was 86.79%, total error rate was 9.38%, and total omission rate was 15.77%. The difference between the detection result and standard result was statistically significant（[χ2] = 12.99, P < 0.01）. Twenty out of 28 counties (districts, cities) had omission detections, accounting for 71.43%; and 13 had fault detections, accounting for 46.43%. The error rates of workers from the river marshland, hilly areas, water networks, and lake marshland ranged from 4.55% to 43.75%, and the difference was statistically significant （[χ2] = 30.34, P < 0.01）. The omission rate ranged from 4.17% to 20.45%, and the difference was not statistically significant （[χ2] = 5.09, P = 0.17）. The error rates and omission rates of workers from the transmission control areas and transmission interruption areas were 7.50%, 13.33% and 10.42%, 17.13%, respectively, and the differences were not statistically significant （[χ2] = 0.229, 0.575, both P > 0.05）. The error rates and omission rates of workers from the areas with or without at least ten years history of transmission control were 11.81%, 5.00% and 16.67%, 14.17%, respectively, and the differences were not statistically significant （[χ2] = 2.804, 2.848, both P > 0.05）. The error rates of workers from the areas with or without at least ten years history of transmission interruption were 11.54% and 10.00%, respectively, and the difference was not statistically significant （[χ2] = 0.069, P = 0.792）, while the correspondent omission rates were 10.90% and 35.00% respectively, and the difference was statistically significant （[χ2] = 17.364, P < 0.01）. Conclusion The detection error and omission exist in the schistosomiasis examinations in the county?level organizations in Jiangsu Province, and therefore, the field pathogen detection capacity of the professional personnel needs to be further improved.

Key words: Schistosomiasis； Diagnosis； Stool hatching method； Quality control； Field assessment