Abstract:

This paper reviews the domestic and foreign literature of toxoplasmosis molecular diagnosis. The nucleic acid molecule hybridization and polymerase chain reaction (PCR) common Toxoplasma gondii gene sequences and conventional PCR, nested PCR, real-time quantitative PCR, immune PCR, in-situ PCR, loop-mediated isothermal amplification technology are introduced. The application of molecular biology technology in toxoplasmosis diagnosis is reviewed.

Key words: Toxoplasmosis, Gene, Molecular diagnosis

摘要：

近年来，弓形虫病分子诊断技术发展较快，本文介绍核酸分子杂交、聚合酶链反应（PCR）常用的弓形虫基因序列，及其常规PCR、巢式PCR、实时定量PCR、免疫PCR、原位PCR、环介导等温扩增等分子生物学技术在弓形虫病诊断中的研究与应用。

关键词: 弓形虫病, 基因, 分子诊断