Abstract: Objective　To investigate the seroprevalence of Toxoplasma gondii infections among patients with diabetes mellitus in Hangzhou City. Methods　A total of 337 patients with type 1 diabetes mellitus, 624 patients with type 2 diabetes mellitus and 384 patients with gestational diabetes mellitus living in Hangzhou City during the period from March 2017 through May 2020 were recruited as the study subjects, while age⁃ and gender⁃matched healthy volunteers and pregnant women without gestational diabetes mellitus served as controls. Enzyme⁃linked immunosorbent assay (ELISA) was employed to detect serum IgG and IgM antibodies against T. gondii in patients with diabetes mellitus and controls, and the seroprevalence of anti⁃T. gondii antibody was compared between diabetes mellitus patients and controls. Results　The overall seroprevalence of anti⁃T. gondii antibody(18.10% vs. 4.45%, [χ2] = 31.38, P < 0.01) and the seroprevalence of anti⁃T. gondii IgG antibody (14.54% vs. 2.97%, [χ2] = 28.28, P < 0.01) were both significantly higher in patients with type 1 diabetes mellitus than in healthy controls, while no significant difference was seen in the seroprevalence of anti⁃T. gondii IgM antibody between patients and controls (3.56% vs. 1.48%, [χ2] = 2.96, P > 0.05). The seroprevalence rates of serum anti⁃T. gondii (23.56% vs. 6.57%, [χ2] = 70.37, P < 0.01) and anti⁃T. gondii IgG antibodies (21.15% vs. 5.45%, [χ2] = 66.73, P < 0.01) were significantly higher in patients with type 2 diabetes mellitus than in healthy controls, while no significant difference was seen in the seroprevalence of anti⁃T. gondii IgM antibody between patients and controls (2.40% vs. 1.12%, [χ2] = 2.96, P > 0.05). In addition, the overall seroprevalence of serum anti⁃T. gondii antibody(26.30% vs. 19.53%, [χ2] = 4.98, P < 0.05) and the seroprevalence of anti⁃T. gondii IgG antibody (23.70% vs. 17.71%, [χ2] = 4.20, P < 0.05) were both significantly higher in patients with gestational diabetes mellitus than in pregnant women without gestational diabetes mellitus, while no significant difference was seen in the seroprevalence of anti⁃T. gondii IgM antibody between pregnant women with and without gestational diabetes mellitus (2.60% vs. 1.82%, [χ2] = 0.54, P > 0.05). Conclusions　The patients with diabetes mellitus present a higher seroprevalence rate of anti⁃T. gondii antibody than controls in Hangzhou City. Screening of T. gondii infections and health education pertaining to toxoplasmosis prevention and control knowledge should be reinforced in patients with diabetes mellitus.

Key words: Toxoplasma gondii, Diabetes mellitus, Seroepidemiological survey, Hangzhou City

摘要： 目的　了解杭州市糖尿病患者弓形虫感染情况。方法　以2017年3月至2020年5月杭州市收治的337例1型糖尿病患者、624例2型糖尿病患者和384例妊娠糖尿病患者作为调查对象，按1∶1的比例分别选择同期年龄和性别相匹配的健康体检者及无妊娠糖尿病孕妇作为对照。采用酶联免疫吸附试验检测糖尿病患者和对照者血清抗弓形虫IgG和IgM抗体，比较糖尿病患者及相应对照血清抗体阳性率差异。结果　杭州市1型糖尿病患者血清抗弓形虫抗体总体阳性率（18.10% vs. 4.45%，[χ2] = 31.38，P < 0.01）和IgG抗体阳性率（14.54% vs. 2.97%，[χ2] = 28.28，P < 0.01）均显著高于健康对照，但两者抗弓形虫IgM抗体阳性率差异无统计学意义（3.56% vs. 1.48%，[χ2] = 2.96，P > 0.05）。2型糖尿病患者血清抗弓形虫抗体总体阳性率（23.56% vs. 6.57%，[χ2] = 70.37，P < 0.01）和IgG抗体阳性率（21.15% vs. 5.45%，[χ2] = 66.73，P < 0.01）均显著高于健康对照，但两者抗弓形虫IgM抗体阳性率差异无统计学意义（2.40% vs. 1.12%，[χ2] = 2.96，P > 0.05）。妊娠糖尿病患者血清抗弓形虫抗体总体阳性率（26.30% vs. 19.53%，[χ2] = 4.98，P < 0.05）和IgG抗体阳性率（23.70% vs. 17.71%，[χ2] = 4.20，P < 0.05）均显著高于健康对照，但两者抗弓形虫IgM抗体阳性率差异无统计学意义（2.60% vs. 1.82%，[χ2] = 0.54，P > 0.05）。结论　杭州市糖尿病患者弓形虫感染血清学阳性率显著高于健康对照，应加强糖尿病患者弓形虫感染筛查和相关健康教育。

关键词: 刚地弓形虫, 糖尿病, 血清流行病学调查, 杭州市