Chin J Schisto Control ›› 2020, Vol. 32 ›› Issue (6): 618-.

### Transcriptome sequencing and bioinformatics analysis of Tyrophagus putrescentiae

JIA Hao-Yuan, ZHOU Ying△, CUI Yu-Bao, WANG Ting-Ting*

1. Department of Clinical Laboratory, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi 214023, China
• Online:2020-12-08 Published:2020-12-08

### 腐食酪螨转录组测序及生物信息学分析

1. 南京医科大学附属无锡人民医院检验科（无锡214023）
• 作者简介:贾浩源，女，博士，主管技师。研究方向：支气管哮喘及其环境因素 周颖，女，本科，副主任技师。研究方向：儿童支气管哮喘与免疫调节
• 基金资助:
江苏省无锡市卫生健康委员会课题（MS201829）；江苏省无锡市卫生和计划生育委员会青年项目（Q201826）

Abstract: Objective　To obtain the transcriptome data of Tyrophagus putrescentiae, so as to provide insights into the subsequent functional studies. Methods　The mixture of male and female T. putrescentiae was sequenced using the Illumina HiSeqTM 2000 high?throughput sequencing platform. Unigenes were obtained after assembling the sequencing data using the Trinity software and compared with the protein sequences in the RefSeq non?redundant protein sequence (NR) database, nucleotide sequence (NT) database, Swiss?Prot database, Kyoto encyclopedia of genes and genomes (KEGG) database and clusters of orthologous groups (COG) database, and the function of the Unigenes was annotated. In addition, the coding DNA sequences (CDS) were predicted through alignment of the Unigenes in NR and Swiss?Prot protein databases. The SSR loci were identified by analysis of the Unigenes in T. putrescentiae with the MISA software, and the SNPs were detected using the SOAPsnp technique. Results　A total of 4.67 GB high?quality data were obtained from raw sequencing data. A total of 51 271 Unigenes were obtained after assembling the sequencing data, with a total length of 41 848 995 nucleotide (nt) and a mean length of 816 nt. A total of 29 053 annotated Unigenes were obtained following comparisons with the public protein databases, and 27 443 CDS were predicted. In addition, there were 23 092 SSR loci and 148 027 SNPs identified. Conclusions　The database of T. putrescentiae transcriptome is created by sequencing, and a large number of T. putrescentiae transcripts are obtained, which provides a basis for the subsequent functional studies of allergy?related genes.

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