Abstract: Objective　To explore the sensitivity of Culex pipiens pallens to common chemical insecticides in the southwestern region of Shandong Province, so as to provide a theoretical basis for the development of reasonable and effective mosquito control measures. Methods　The resistance of Cx. pipiens pallens larvae to 5 chemical insecticides, such as cypermethrin, deltamethrin, DDVP, propoxur, and acetofenate were tested by using the WHO biological test method in 2018, and the co?toxicity coefficients after compounding the above?mentioned insecticides were tested by using a drug compounding method. Results　The resistance indexes of Cx. pipiens pallens to cypermethrin, deltamethrin, DDVP, propoxur, and acetofenate in 3 cities were 144.43–557.54, 118.17–445.33, 6.44–19.00, 2.37–8.10, and 0.88–2.98, respectively, and expect the difference between the DDVP resistances of Cx. pipiens pallens in Jining City and Heze City was not statistically significant (P > 0.05), all the other differences were statistically significant (all P < 0.05). The synergistic coefficients of cypermethrin + DDVP, cypermethrin + propoxur, DDVP + acetofenate, and propoxur + acetofenate were 199.58 – 456.95, 190.56 – 292.37, 123.32 – 319.24, and 192.31 – 367.32, respectively. The lower synergism was observed by using the mixture of DDVP + propoxur (synergistic coefficient: 99.87–108.36）. Conclusions　After decades of chemical control, Cx. pipiens pallens in the southwestern region of Shandong Province has produced different degrees of resistance to common chemical insecticides. Therefore, comprehensive control measures should be taken to control mosquito breeding and prevent the development of insecticide resistance. 　

Key words: Culex pipiens pallens, Chemical insecticide, Cypermethrin, Deltamethrin, DDVP, Propoxur, Acetofenate, Insecticide mixture, Resistance

摘要： 目的　掌握鲁西南地区现场淡色库蚊对常用化学杀虫剂的抗药性，为制定合理有效的蚊虫防治措施提供参考。方法　采用WHO生物测试法与药物复配法，2018年6-9月测试山东省西南地区济宁市、菏泽市、枣庄市淡色库蚊幼虫对氯氰菊酯、溴氰菊酯、敌敌畏（DDVP）、残杀威、三氯杀虫酯5种化学杀虫剂的抗性，以及杀虫剂混合后的共毒系数。结果　3个地区淡色库蚊对氯氰菊酯、溴氰菊酯、DDVP、残杀威、三氯杀虫酯的抗性倍数分别为144.43～557.54、118.17～445.33、6.44～19.00、2.37～8.10、0.88～2.98，除济宁与菏泽地区淡色库蚊对DDVP的抗性差异无统计学意义（P > 0.05）外, 其他差异均有统计学意义（P均 < 0.05）。氯氰菊酯 + DDVP、氯氰菊酯 + 残杀威、DDVP + 三氯杀虫酯、残杀威 + 三氯杀虫酯复配后共毒系数分别为199.58～456.95、190.56～292.37、123.32～319.24、192.31～367.32，DDVP + 残杀威复配效果不佳（共毒系数：99.87～108.36）。结论　经过几十年的化学防治，鲁西南地区淡色库蚊对常用化学杀虫剂均表现出不同程度的抗性；应采取综合防治措施控制蚊虫孳生，以延缓抗药性的发展。

关键词: 淡色库蚊, 化学杀虫剂, 氯氰菊酯, 溴氰菊酯, DDVP, 残杀威, 三氯杀虫酯, 杀虫剂复配, 抗药性