Chin J Schisto Control ›› 2019, Vol. 31 ›› Issue (3): 285-.

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Study on pathogenicity of Pneumocystis and its association with development of chronic obstructive pulmonary disease

XUE Ting1, MA Su-Li2, HE Li1, AN Chun-Li1*   

  1. 1 Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang, 110122, China; 2 Department of Nursing, School of Medicine, Pingdingshan College, China
  • Online:2019-08-28 Published:2019-08-28

肺孢子菌致病性及其与慢性阻塞性肺疾病(COPD) 相关性研究


  1. 1中国医科大学基础医学院病原生物学教研室(沈阳110122);2平顶山学院护理系
  • 作者简介:薛婷,女,博士研究生。研究方向:机会性真菌分子流行病学与致病性
  • 基金资助:

Abstract: Objective To investigate the pathogenicity?of Pneumocystis and its association with the development of chronic obstructive pulmonary disease (COPD). Methods The rat model of Pneumocystis pneumonia (PCP) was induced by intraperitoneal injection with dexamethasone, which was confirmed by pathogenic detection. The pathologic changes of rat lung specimens were examined using conventional HE staining, and the expression of inflammatory cells were detected by flow cytometry in bronchoalveolar lavage fluid (BALF) and splenic tissues of the rat model of PCP. In addition, the serum levels of matrix metalloproteinase 8 (MMP?8) and MMP?9 were measured using enzyme?linked immunosorbent assay (ELISA). Results Fusion and atrophy of alveolar spaces and hyperplasia of lung tissue were seen in the lung specimens of the rat model of PCP, and foam?like alveolar exudates and infiltration of inflammation cells were observed in the alveolar space, while severe infections exhibited consolidation of lung, which was similar to pathological features of COPD. The counts of CD8+ T lymphocytes (t = -7.920 and -12.514, P < 0.01), macrophages (t = -7.651 and -14.590, P < 0.01) and granulocytes (t = -10.310 and -16.578, P < 0.01) significantly increased and the counts of CD4+ T lymphocytes (t = 6.427 and 18.579, P < 0.01) significantly reduced in the BALF and splenic specimens of the rats with PCP relative to those without PCP. In addition, higher serum MMP?8 (t = -8.689, P < 0.01) and MMP?9 levels (t = -7.041, P < 0.01) were measured in rats with PCP than in those without PCP. Conclusion Pneumocystis infection may be associated with the development and progression of COPD.

Key words: Pneumocystis, Pneumocystis pneumonia, Chronic obstructive pulmonary disease, Pathogenicity, Correlation study

摘要: 目的 研究肺孢子菌的致病性及其与慢性阻塞性肺疾病(COPD)的相关性。方法 用免疫抑制剂诱导大鼠感染肺孢子菌建立肺孢子菌肺炎(PCP)大鼠模型。常规病理切片HE染色镜检观察大鼠肺组织病理学改变,采用流式细胞术检测PCP大鼠肺泡灌洗液(BALF)和脾脏中炎性细胞水平、酶联免疫吸附试验(ELISA)检测PCP大鼠血清中基质金属蛋白酶?8(MMP?8)和MMP?9表达水平。结果 PCP大鼠肺组织出现肺泡腔融合、萎陷,肺组织增生,肺泡腔内有泡沫样渗出物并有炎性细胞浸润,严重者出现肺组织实变等与COPD相似的病理形态学特征;与对照组相比,PCP大鼠BALF和脾组织中CD8+ T淋巴细胞(BALF:t = -7.920,脾组织:t = -12.514,P均< 0.01)、巨噬细胞(BALF:t = -7.651,脾组织:t = -14.590,P均< 0.01)、粒细胞(BALF:t = -10.310,脾组织:t = -16.578,P均< 0.01)均明显上升,CD4+ T淋巴细胞(BALF:t = 6.427,脾组织:t = 18.579,P均< 0.01)明显下降;PCP大鼠血清中MMP?8(t = -8.689,P < 0.01)和MMP?9(t = -7.041,P < 0.01)水平均显著高于对照组。结论 肺孢子菌感染可能与COPD发生发展存在一定相关性。

关键词: 肺孢子菌, 肺孢子菌肺炎, 慢性阻塞性肺疾病, 致病性, 相关研究

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