Chinese Journal of Schistosomiasis Control ›› 2022, Vol. 34 ›› Issue (4): 370-.

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Prevalence and risk factors of Giardia lamblia infections among colorectal cancer patients in Henan Province

CHEN Hui⁃hui1△, DENG Yan2△, LI Zhi3, WANG Zhen⁃lei3, RUN Zeng⁃ci3, ZHANG Ting1, CAI Yu⁃chun1, ZHANG Hong⁃wei2, HU Zhu⁃hua4, CHEN Jun⁃hu1, TIAN Li⁃guang1*, LI Jian3*   

  1. 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, (Chinese Center for Tropical Diseases Research), National Health Commission Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, School of Global Health, Chinese Center for Tropical Diseases and Shanghai Jiaotong University School of Medicine, Shanghai 200025, China; 2 Henan Provincial Center for Disease Control and Prevention, Zhengzhou, Henan 450016, China; 3 Henan Cancer Hospital, Zhengzhou, Henan 450003, China; 4 Research Base of National Key Laboratory of Infectious Disease Prevention and Control, Key Laboratory of Animal Origin and Vector⁃borne Infectious Diseases of Jiangxi Province, Nanchang Center for Disease Control and Prevention, Jiangxi Province, China
  • Online:2022-08-31 Published:2022-08-31

河南省结直肠癌患者蓝氏贾第鞭毛虫感染及危险因素分析

陈慧慧1△,邓艳2△,李智3,王振雷3,润增慈3,张颋1,蔡玉春1,张红卫2,胡主花4,陈军虎1, 田利光1*,李剑3*   

  1. 1 中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、WHO热带病合作中心、科技部国家级热带病国际研究中心、上海交通大学医学院⁃国家热带病研究中心全球健康学院(上海 200025);2 河南省疾病预防控制中心(河南 郑州 450016);3 河南省肿瘤医院(河南 郑州 450003);4 传染病预防控制国家重点实验室研究基地、江西省动物源与媒介生物性传染病重点实验室、江西省南昌市疾病预防控制中心
  • 作者简介:陈慧慧,女,硕士研究生。研究方向:寄生虫病流行病学 邓艳,女,硕士,副主任医师。研究方向:寄生虫病防治
  • 基金资助:
    上海市自然科学基金(21ZR1469900);WHO示范项目(UNOPS/ANDI/G/2016/01);江西省南昌市科技支撑重点项目(洪科字[2021]129号)

Abstract: Objective To investigate the epidemiological characteristics and identify the risk factors of Giardia lamblia infections among patients with colorectal cancer in Henan Province. Methods A cross⁃sectional study was performed for questionnaire surveys among colorectal cancer patients in Henan Cancer Hospital during the period from March to July, 2021. Patients’ stool samples were collected, and the triosephosphate isomerase (tpi) gene of G. lamblia was amplified in stool samples using nested PCR assay to characterize the parasite genotype. Univariate analysis and multivariate logistic regression analyses were employed to identify the risk factors of G. lamblia infections among colorectal cancer patients. Results A total of 307 colorectal cancer patients were investigated, including 176 males (57.3%) and 131 females (42.7%). PCR assay detected 8.1% [95% confidential interval (CI): (0.056, 0.117)] prevalence of G. lamblia infections among the study subjects, and there was no significant difference in the prevalence between men [9.1%, 95% CI: (0.057, 0.143)] and women [6.9%, 95% CI: (0.037, 0.125)] ([χ2] = 0.495, P = 0.482). In addition, there was no age⁃specific prevalence of G. lamblia infections among the participants ([χ2] = 1.534, P = 0.675). Multivariate logistic regression analysis identified use of septic tanks [odds ratio (OR) = 3.336, 95% CI: (1.201, 9.267)], daily use of well water [OR = 3.042, 95% CI: (1.093, 8.465)] and raising livestock [OR = 3.740, 95% CI: (1.154, 12.121)] as risk factors of G. lamblia infections among colorectal cancer patients, and the prevalence of abdominal pain was significantly greater in colorectal cancer patients with G. lamblia infections than in those without infections (P = 0.017). Among the 25 patients with G. lamblia infections, assemblage A was characterized in 24 (96.0%) cases and assemblage B in one case (4.0%). Conclusions  The prevalence of G. lamblia is high among colorectal cancer patients in Henan Province, and assemblage A is the dominant genotype of G. lamblia. Use of septic tanks, daily use of well water and raising livestock are risk factors of G. lamblia infections among patients with colorectal cancer.

Key words: Giardia lamblia, Colorectal cancer, Genotype, Risk factor, Henan Province

摘要: 目的 了解河南省结直肠癌患者蓝氏贾第鞭毛虫感染特征并分析其危险因素。方法 2021年3—7月,采用横断面调查法对河南省肿瘤医院结直肠癌患者进行问卷调查,并收集其粪便样本,采用巢式PCR法扩增蓝氏贾第鞭毛虫磷酸丙糖异构酶(tpi)基因以确定感染虫种基因型。采用单因素分析和多因素logistic回归模型分析结直肠癌患者感染蓝氏贾第鞭毛虫的危险因素。结果 共调查结直肠癌患者307例,其中男性176例(占57.3%)、女性131例(占42.7%)。巢式PCR检测粪样基因组DNA发现,蓝氏贾第鞭毛虫感染率为8.1%[25/307,95% 可信区间(CI):(0.056,0.117)],其中男性感染率为9.1%[16/176,95% CI:(0.057,0.143)]、女性感染率为6.9%[9/131,95% CI:(0.037,0.125)]([χ2] = 0.495,P = 0.482)。不同年龄组结直肠癌患者蓝氏贾第鞭毛虫感染率差异无统计学意义([χ2] = 1.534,P = 0.675)。多因素分析发现,使用粪池[比值比(OR)= 3.336,95% CI:(1.201,9.267)]、日常使用水井水[OR = 3.042,95% CI:(1.093,8.465)]及饲养家畜[OR = 3.740,95% CI:(1.154,12.121)]是结直肠癌患者感染蓝氏贾第鞭毛虫的危险因素。结直肠癌患者中,蓝氏贾第鞭毛虫感染者腹痛发生率显著高于非感染者(P = 0.017)。25例蓝氏贾第鞭毛虫感染者中,24例(占96.0%)为聚集体A型感染、1例(占4.0%)为聚集体B型感染。结论 河南省结直肠癌患者蓝氏贾第鞭毛虫感染率相对较高,感染的蓝氏贾第鞭毛虫主要基因型为聚集体A型;使用粪池、日常使用水井水及饲养家畜是结直肠癌患者感染蓝氏贾第鞭毛虫的危险因素。  

关键词: 蓝氏贾第鞭毛虫, 结直肠癌, 基因型, 危险因素, 河南省

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