Chin J Schisto Control ›› 2020, Vol. 32 ›› Issue (1): 15-.

### Diagnostic value of nucleic acid detection in schistosomiasis japonica: a meta-analysis

WANG Sheng-Lin, WANG Li-Ping, WU Ling-Ling, LI Yin-Long, ZHANG Li-Juan, Lü Shan, XU Jing*

1. National Institute of Parasitic Diseases， Chinese Center for Disease Control and Prevention； WHO Collaborating Centre for Tropical Diseases； Key Laboratory of Parasites and Vector Biology of National Health Commission； Chinese Center for Tropical Diseases Research， Shanghai 200025， China
• Online:2020-03-03 Published:2020-03-03

### 核酸检测技术对日本血吸虫病诊断价值的meta分析

1. 中国疾病预防控制中心寄生虫病预防控制所、WHO热带病合作中心、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、国家热带病研究中心（上海200025）
• 作者简介:王盛琳，女，硕士研究生。研究方向：血吸虫病流行病学
• 基金资助:
国家科技重大专项（2018ZX10101002?002、2016ZX10004222?004）

Abstract: Objective　To quantitatively evaluate the diagnostic value of variable?temperature and isothermal nucleic acid amplification techniques in the detection of schistosomiasis japonica using a meta?analysis. Methods　The publications pertaining to the nucleic acid detection of schistosomiasis japonica were searched in electronic databases, including Chinese National Knowledge Infrastructure (CNKI), Wanfang Data, PubMed and ScienceDirect, and the compilations and proceedings of schistosomiasis were manually searched. In addition, the citations of publications associated with the nucleic acid detection of schistosomiasis japonica were traced using a document tracing method. The retrieved literatures were screened according to the inclusion and exclusion criteria, and data were extracted from the included literatures. The quality of the included literatures was assessed using the software RevMan version 5.3, and a meta?analysis was performed using the software MetaDiSc version 1.4. Results　A total of 19 publications covering 24 groups of studies were enrolled, including 5 Chinese publications and 14 English publications. There were 17 groups of studies reporting the comparison between the variable?temperature nucleic acid amplification technique and the golden standard, and 7 groups of studies showing the comparison between the isothermal nucleic acid amplification technique and the golden standard. Assessment of the literature quality indicated a minor overall bias of the included literatures, and the Deek funnel plot showed a possible publication bias in the documents reports variable?temperature nucleic acid amplification techniques. There was a heterogeneity caused by non?threshold effect among the studies associated with the variable?temperature amplification technique, and the random effects model was therefore used to combine the effects. The pooled sensitivity and specificity of the variable?temperature amplification technique were 0.81 (0.79 to 0.83) and 0.73 (0.71 to 0.74) for the diagnosis of schistosomiasis japonica, and area under the SROC curve was 0.944 3. There was no heterogeneity among the studies associated with the isothermal amplification technique, and the fixed effects model was therefore used to combine the effects. The pooled sensitivity and specificity of the isothermal amplification technique were 0.96 (0.94 to 0.98) and 0.95 (0.94 to 0.97) for the diagnosis of schistosomiasis japonica, and area under the SROC curve was 0.989 9. Conclusion　Both variable?temperature and isothermal nucleic acid amplification techniques have a high efficiency for the diagnosis of schistosomiasis japonica, and the isothermal amplification technique shows a relatively higher accuracy than the variable?temperature amplification technique.

CLC Number: