Abstract:

Objective To investigate the molecular characteristics of genome sequence of Thelazia callipaeda （T. cp）. Methods The obtained T. cp genome assembling data were annotated by using a combination of ab initio gene by softwares， GeneMark and GeneID， and the homology of the experimentally confirmed genes was predicted by software GeMoMa. The results were integrated by software EVM to predict all genes of genome. The obtained genes were annotated in the common public database and three dedicated databases（CAZyme， TCDB and PHI）， respectively. Results The Scaffolds and Contigs gene structure of T. cp genome （79.34 Mb） was analyzed， and a total of 6 333 genes were obtained. The sequence search was conducted in the public databases using BLASTx， of which 97.85% of the genes could be annotated. The genes annotated in the NR database were the most （98.69%）， and those enriched in the KEGG pathway were the least （50.50%）. The functional genes were blasted by KOG database and totally 4 517 genes were found. The three special databases （CAZyme， TCDB and PHI） were used to annotate all the genes， and 136， 139 and 1 498 genes were assigned respectively， and the number of genes in the PHI database was the largest. In the cytochrome proprietary database， 238 cytochrome P450 genes were predicted. Conclusion We have preliminarily revealed the T. cp genome structure characteristics and annotation information， and totally 6 333 genes are obtained.

Key words: Thelazia callipaeda； Genome； Sequence analysis； Functional gene； Cytochrome P450

摘要：

目的 阐明结膜吸吮线虫（Thelazia callipaeda）基因组的序列特征。方法 采用GeneMark、GeneID和GeMoMa软件对结膜吸吮线虫基因组组装数据进行从头预测及同源预测，利用EVM软件对预测结果进行整合，以预测其基因组全部基因；将得到的基因序列分别在公共数据库及3个专有数据库（CAZyme、TCDB和PHI）中进行注释。结果 对结膜吸吮线虫基因组（79.34 Mb）的Scaffolds和Contigs基因结构进行分析，共得到了6 333个基因；通过公共数据库中BLAST比对，发现97.85%的基因可以得到注释，其中NR数据库中注释的基因最多（98.69%），可以富集到KEGG途径的基因最少（50.50%）。通过KOG数据库分析功能基因，共发现4 517个功能基因。在3个专有数据库（CAZyme、TCDB和PHI）中比对，分别注释得到136、139个和1 498个基因，其中PHI数据库中注释基因数目最多（1 498）。此外，还通过细胞色素酶的专有数据库预测到了238个细胞色素P450基因。结论 本研究初步揭示了结膜吸吮线虫基因组结构特征和注释信息，共得到了6 333个基因。

关键词: 结膜吸吮线虫；基因组；序列分析；功能基因；细胞色素P450