Chin J Schisto Control ›› 2016, Vol. 28 ›› Issue (2): 141-145.

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Investigation of two blood parasitic protozoa infection in farmed Macaca fascicularis in Guangxi Zhuang Autonomous Region

LU Yuan-yuan1, 2 ,PENG Heng2 ,ZHU Huai-min2* , LI Jian3 ,XUE Shao-li 4*   

  1. 1 School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China;2 Department of Medical Microbiology and Parasitology,Second Military Medical University,China;3 School of Life Sciences,Fudan University,China;4 Department of Biological Engineering, Anhui Medical University,China
  • Online:2016-04-19 Published:2016-04-20
  • Contact: ZHU Huai?min

我国广西食蟹猴养殖场2种血液寄生原虫感染调查

路媛媛1,2,彭恒2,朱淮民2*,李健3,薛绍礼4*   

  1. 1 安徽医科大学基础医学院 (合肥 230032);2 第二军医大学病原生物学教研室;3 复旦大学生命科学院;4 安徽医科大学生物工 程系
  • 通讯作者: 朱淮民
  • 作者简介:路媛媛, 女, 硕士研究生。研究方向: 寄生虫检测以及诊断方法
  • 基金资助:
    国家科技重大专项 (2012ZX10004?220); 军队后勤科研项目 (CWS12BJ06); 卫生行业科研专项经费 (201202019)

Abstract: Objective Objective To investigate the infection situation of blood parasitic protozoa in farmed Macaca fascicularis in an animal breeding ground in Nanning,Guangxi Zhuang Autonomous Region,so as to provide the evidence for the prevention and control of human blood parasitic protozoa. Methods Methods A total of 993 blood samples from farmed M. fascicularis were collected and stored on FTA cards. Among them,550 thin blood smears were made. Each 10 samples were mixed in groups,and then the Babesia spp. and Plasmodium spp. in the blood of M. fascicularis were detected by Nest?PCR and PCR,respectively. The posi? tive groups were tested individually. The thin blood smears stained with Giemsa were examined microscopically when PCR re? ported the samples were positive. Results Results When detected by Nest?PCR,the positive rate of Babesia. microti was 6.95% (69/993);only 1 positive sample with Plasmodium inui was detected by PCR. Among the 22 positive thin blood smears detect? ed by PCR,16 were determined with B. microti by microscopic examinations,on which the ring forms could be observed in the erythrocytes,but no hemozoin. Conclusions Conclusions The positive rate of B. microti in M. fascicularis in Guangxi Zhuang Autonomous Region is high,and the animal may play a role as a reservoir host in the transmission of B. microti. In the screening of B. microti with low infection density, Nest?PCR has a higher sensitivity.

Key words: Macaca fascicularis, Babesia microti, Plasmodium inui, Reservoir host, Guangxi Zhuang Autonomous Re? gion

摘要: 目的 目的 调查我国广西壮族自治区某食蟹猴养殖场血液寄生原虫感染状况, 为人体血液寄生原虫的防控提供科 学依据。方法 方法 采集广西壮族自治区南宁市某食蟹猴养殖场猴血液样本993份, 全部制作FTA卡样本, 涂制薄血膜550 份。将样本混合, 以巢式PCR及普通PCR方法分别检测FTA卡保存的猴血样本中巴贝虫以及疟原虫。检测阳性组再分 别进行单个样本检测, 阳性样本对应的薄血膜进行姬姆萨染色后再镜检。 结果 结果 经巢式PCR检测, 田鼠巴贝虫检出阳 性率为6.95% (69/993); 仅1例经PCR检出猪尾猴疟原虫阳性。22份PCR检测巴贝虫阳性的血液样本经染色镜检, 共16 份检出巴贝虫, 其显微镜下观察到红细胞内有环状体, 无疟色素。结论 结论 我国广西地区食蟹猴的田鼠巴贝虫感染率较 高, 其可能在传播中起保虫宿主的作用; 在筛查田鼠巴贝虫低密度感染时, 巢式PCR方法敏感性较高。

关键词: 食蟹猴, 田鼠巴贝虫, 猪尾猴疟原虫, 保虫宿主, 广西壮族自治区

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