Abstract: Objective Objective To observe the effect of artificially construction of the life cycle of Angiostrongylus cantonensis in the laboratory condition，so as to provide the basis for the research of angiostrongyliasis. Methods Methods SD rats were infected orally with the third?stage larvae of A. cantonensis collected from infected Pomacea canaliculata. Six weeks after the infection， the first? stage larvae were isolated and counted from fresh feces of the rats and then were used to infect P. canaliculata. Three weeks lat? er，the snails were dissected for counting the third?staged larvae of A. cantonensis. Results Results The first?stage larvae were detect? ed in the feces of the rats 6 weeks after the infection，and the third?staged larvae were successfully isolated after the infection of P. canaliculata. Conclusion Conclusion The animal model of the entire life cycle of A. cantonensis is successfully established in the labo? ratory with the infection of 50 larvae per rat.

Key words: Angiostrongylus cantonensis； Life cycle； Pomacea canaliculata； SD rat；Animal model

摘要： 目的 目的 观察人工建立广州管圆线虫生活史动物模型的效果， 为进一步开展广州管圆线虫病研究提供基础资 料。 方法 方法 从人工感染广州管圆线虫的福寿螺中分离出3期幼虫， 经灌胃感染SD大鼠。感染后第6周从大鼠粪便中分 离、 计数广州管圆线虫1期幼虫， 并将大鼠每天排出的1期幼虫人工感染健康福寿螺。3周后， 用胃蛋白酶消化法， 从福 寿螺内分离出广州管圆线虫3期幼虫。 结果 结果 SD大鼠感染广州管圆线虫3期幼虫后第6周， 其粪便中均检出1期幼虫， 并成功感染健康福寿螺得到3期幼虫。 结论 结论 在实验室条件下以每只大鼠50条广州管圆线虫3期幼虫的感染量可成功 建立广州管圆线虫生活史动物模型及其实验室种群。

关键词: 广州管圆线虫； 生活史； 福寿螺； SD大鼠； 动物模型