Abstract: Objective Objective To isolate Toxoplasma gondii（T. gondii）strains from stray cats and explore their prevalence in Xu? zhou City. Methods Methods The sera of 41 stray cats were collected to detect the antibodies of T. gondii by using a commercial enzyme? linked immunosorbent（ELISA）kit. The tissues including the heart，brain and tongue from these cats were digested by acid pep? sin solution and inoculated to Kunming mice. These suspicious isolates were subsequently identified by a specific PCR method. Results Results A total of 11 strains were isolated from 41 stray cats，which were confirmed by the PCR results. Moreover，17 cats （41.5%）were found to be positive with the antibodies of T. gondii. Conclusion Conclusion A high prevalence of T. gondii infection was found in Xuzhou City，which indicates that the stray cats infected with T. gondii would be an important infection source that may infect humans and other animals in this area.

Key words: Toxoplasma gondii；Stray cat； Isolation and identification；Xuzhou region

摘要： 目的 目的 获得徐州地区猫源弓形虫虫株， 了解徐州地区流浪猫弓形虫感染情况。方法 方法 捕捉徐州地区流浪猫， 收 集血清后， 用ELISA试剂盒检测弓形虫特异性IgG抗体； 同时将猫的心、 脑、 舌用盐酸?胃蛋白酶溶液消化处理后接种小 鼠， 经连续传代分离弓形虫虫株， 采用特异PCR法对所分离的虫株进行鉴定。结果 结果 共捕捉41只流浪猫。从猫体内共 分离到弓形虫虫株11株， PCR均扩增出弓形虫特异性条带； ELISA结果表明41.5%的流浪猫均能检测到弓形虫抗体。结 结 论 论 徐州地区流浪猫弓形虫感染较为严重， 有可能成为人和其它动物弓形虫病的重要传染源， 应引起相关部门的重视。

关键词: 弓形虫； 流浪猫； 分离与鉴定； 徐州地区