Abstract: Objective Objective To investigate the relationship between p53，COX?2，Bax，c ?myc genes and colorectal carcinoma complicated with chronic schistosomiasis. Methods Methods One hundred and sixty patients with colorectal carcinoma were selected and divided into two groups；a schistosomiasis group（colorectal carcinoma complicated with chronic schistosomiasis，n = 80）and a non?schistosomiasis group（colorectal carcinoma uncomplicated with chronic schistosomiasis，n = 80） . The tissue microarray tech? niques and immunohistochemistry method were used in all the patients to detect the expressions of p53，COX?2，Bax and c?myc proteins. Results Results The positive rate and level of p53 protein expression in the schistosomiasis group were lower than those in the non?schistosomiasis group，but there were no significant differences between the two groups（both P > 0.05） . The COX?2 protein in both groups was positive，but the positive expression level of COX?2 in the schistosomiasis group was higher than that in the non? schistosomiasis group，and there was a significant difference between the two groups（P < 0.01） . The positive rate and level of Bax protein expression were not significantly different between the two groups（both P > 0.05） . The positive rate of c?myc expression in the schistosomiasis group was higher than that in the non?schistosomiasis group，with a significant difference（P < 0.01），but the positive expression level was lower than that in the non?schistosomiasis group，and there was a significant difference between the two groups（P < 0.01） . Conclusions Conclusions Schistosome infection may impact on the deficiency of p53 of human colorectal cancer cells. It may promote the excessive expression of COX?2 protein，which is an indirect carcinogenic factor. The expression of Bax gene has no correlation with schistosome infection. The schistosome chronic infection may cause a persistent low level expression of c? myc gene.

Key words: Schistosomiasis； Colorectal carcinoma； p53；COX?2； Bax； c?myc；Tissue microarray

摘要： 目的 目的 探讨血吸虫病合并大肠癌与p53、 环氧合酶?2 （COX?2）、 Bax、 c?myc蛋白表达间的相关性。方法 方法 160例大 肠癌患者分为2组： 血吸虫病组 （慢性血吸虫病合并大肠癌） 80例和非血吸虫病组 （单纯性大肠癌） 80例。采用组织芯片及 免疫组化EnVision法检测， 比较2组患者中p53、 COX?2、 Bax、 c?myc蛋白的表达水平。结果 结果 p53蛋白在血吸虫病组的阳性 表达率与阳性表达水平均低于非血吸虫病组， 但差异均无统计学意义 （P 均> 0.05） ； COX?2蛋白在两组中均为阳性表达， 血 吸虫病组表达水平显著高于非血吸虫病组 （P＜0.01）； 两组Bax蛋白阳性表达率与阳性表达水平差异均无统计学意义 （P 均 > 0.05） 。血吸虫病组c?myc蛋白阳性表达率显著高于非血吸虫病组， 但阳性表达水平显著低于非血吸虫病组 （P均 ＜0.01）。结论 结论 血吸虫感染可能对大肠癌组织细胞中p53缺失有一定的影响， 对COX?2蛋白的过表达有促进作用， 是一 个间接致癌因素； Bax与血吸虫感染无相关性； 慢性血吸虫感染可能引起c?myc蛋白的持续性低水平表达。

关键词: 血吸虫病； 大肠癌； p53； COX?2； Bax； c?myc； 组织芯片